Column-switching high-performance liquid chromatographic analysis of fluvastatin in rat plasma by direct injection

So Young Um, Sung Hee Jung, Seo Jeong Jung, Joo Il Kim, Soo Youn Chung, Hwa Jeong Lee, Sang Beom Han, Sun Ok Choi

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A column-switching high-performance liquid chromatographic (HPLC) method has been developed and validated for quantification of fluvastatin in rat plasma. Plasma samples were diluted with an equal volume of mobile phase, i.e. acetonitrile-5 mM potassium phosphate buffer (pH 6.8) (15:85, v/v), and the mixture was directly injected onto the HPLC system. The analyte was enriched in a pre-treatment column, while endogenous components were eluted to waste. The analyte was then back-flushed onto an analytical column and quantified with fluorescence detection (λex = 305 nm; λem = 390 nm). The standard curve for the drug was linear in the range 0.5-100 ng mL-1 in rat plasma. The limit of quantitation for plasma was found to be 0.5 ng mL-1. This method has been fully validated and shown to be specific, accurate and precise. The method is simple and rapid because of a minimized sample preparation and appears to be useful for the pharmacokinetic study of fluvastatin.

Original languageEnglish
Pages (from-to)1458-1462
Number of pages5
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume41
Issue number4
DOIs
StatePublished - 16 Jun 2006

Keywords

  • Column-switching
  • Direct injection
  • Fluvastatin
  • High-performance liquid chromatography (HPLC)
  • Rat plasma

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