Colorimetric and fluorometric assays based on conjugated polydiacetylene supramolecules for screening acetylcholinesterase and its inhibitors

Guodong Zhou, Fang Wang, Huilin Wang, Srinivasulu Kambam, Xiaoqiang Chen, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Polydiacetylene supramolecules (PDAs) are unique sensing materials. Upon environmental stimulation, blue PDAs can undergo a colorimetric transition from blue to red accompanied by fluorescence enhancement. In this paper, we report a new PDA system polymerized from a mixed liposome comprising 2-(2-(2- hydroxyethoxy)ethoxy)ethyl pentacosa-10,12-diynoate and pentacosa-10,12-diynoic acid at a 3:7 ratio. The PDA system provided new colorimetric and fluorometric assay methods for screening acetylcholinesterase and its inhibitors through three processes. First, myristoylcholine reacted with PDAs, which then underwent colorimetric and fluorometric transition. Second, acetylcholinesterase catalyzed the hydrolysis of myristoylcholine into tetradecanoic acid, which reduced the myristoylcholine concentration and led to faded color and fluorescence. Third and last, acetylcholinesterase inhibitors retarded the activity of acetylcholinesterase, thereby inducing the recovery of color and fluorescence.

Original languageEnglish
Pages (from-to)3275-3280
Number of pages6
JournalACS Applied Materials and Interfaces
Volume5
Issue number8
DOIs
StatePublished - 24 Apr 2013

Keywords

  • acetylcholinesterase
  • colorimetric and fluorometric assays
  • conjugated polymer
  • inhibitor
  • myristoylcholine
  • polydiacetylene

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