The melanoidin from soluble coffee is utilized as a material-independent, multipurpose coating material. Instantaneous complexation of the coffee melanoidin (CM) with ferric ion (Fe3+) leads to surface-adhesive aggregates, inducing sequential film deposition. Various chemical groups of the CM also allow for post-functionalizations of the CM film, including surface-initiated, ring-opening polymerization and bioinspired silicification. In addition, the CM-based coating is applied to single-cell nanoencapsulation with a strategy of biphasic interfacial reactions. The method is highly cytocompatible (viability >98%), and the CM shell is cytoprotective against lytic enzymes. The coated cells inherit the characterictics of the CM, such as post-functionalizability and antioxidant property. Considering that surface-coating technologies with cytocompatible natural polymers have widely been used for engineering bioentities, the CM-based coating strategy would provide an advanced option for biomedical applications.
Bibliographical noteFunding Information:
This work was supported by the Nano Material Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2009-0082580), and developed within the scope of the project CICECO-Aveiro Institute of Materials, FCT Ref. UID/CTM/50011/2019, financed by national funds through the FCT/MCTES. And we would like to thank the UA and FCT/MCT for the financial support for the QOPNA research Unit (FCT UID/QUI/00062/2019) through national founds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement, and to the Portuguese NMR Network. Cl?udia Passos contract (CEECIND/01873/2017) was supported by FCT.
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- cell encapsulation
- coffee melanoidin
- film formation
- surface engineering