Cofactor specificity engineering of a long-chain secondary alcohol dehydrogenase from: Micrococcus luteus for redox-neutral biotransformation of fatty acids

Eun Ji Seo, Hye Ji Kim, Myeong Ju Kim, Jeong Sun Kim, Jin Byung Park

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Structure-based engineering of a NAD+-dependent secondary alcohol dehydrogenase from Micrococcus luteus led to a 1800-fold increase in catalytic efficiency for NADP+. Furthermore, the engineered enzymes (e.g., D37S/A38R/V39S/T15I) were successfully coupled to a NADPH-dependent Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 for redox-neutral biotransformations of C18 fatty acids into C9 chemicals.

Original languageEnglish
Pages (from-to)14462-14465
Number of pages4
JournalChemical Communications
Volume55
Issue number96
DOIs
StatePublished - 2019

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