Cloning and sequence analysis of the rat tumor necrosis factor-encoding genes

Jongbum Kwon, Yup Chung, Etty N. Benveniste

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

We have isolated an approximately 22-kb TNF locus (encoding tumor necrosis factor) from a rat genomic library and sequenced the 7105-bp fragment that comprises the TNF-α and TNF-β genes, including their flanking sequences. The two genes are tandemly arranged with TNF-β 5' to TNF-α and separated by approximately 1.1 kb of intergenic space, and each gene consists of four exons and three introns, similar to those of the other species examined thus far. Comparison analysis showed that the rat TNF have high sequence homology with the mouse TNF (TNF-α, 86.5%; TNF-β, 89.3%) and relatively low homology with the human, rabbit, and porcine TNF. The upstream sequence of rat TNF-α. contains a number of sequence motifs implicated in the expression and regulation of eukaryotic genes, including binding sites for the transcription factors Sp-1, Ap-2, IFN.l and NF-KB. The possible significance of potential regulatory sequence elements found in the rat TNF-α in the context of transcriptional regulatory mechanisms is discussed.

Original languageEnglish
Pages (from-to)227-236
Number of pages10
JournalGene
Volume132
Issue number2
DOIs
StatePublished - 15 Oct 1993

Keywords

  • Astrocytes
  • nuclear factor-KB
  • sequence comparison
  • transcriptional regulation

Fingerprint

Dive into the research topics of 'Cloning and sequence analysis of the rat tumor necrosis factor-encoding genes'. Together they form a unique fingerprint.

Cite this