TY - JOUR
T1 - Cloning and functional characterization of the mouse fructose transporter, GLUT5
AU - Corpe, Christopher P.
AU - Bovelander, Floris J.
AU - Munoz, Christina M.
AU - Hoekstra, J. Hans
AU - Simpson, Ian A.
AU - Kwon, Oran
AU - Levine, Mark
AU - Burant, Charles F.
N1 - Funding Information:
This work was partially supported by the National Institute of Diabetes and Digestive and Kidney Diseases Grant DK-02170 (C.F.B.).
PY - 2002/6/7
Y1 - 2002/6/7
N2 - Mouse GLUT5 cDNA and a 7.7-kb genomic fragment have been isolated and characterized. The cDNA sequence suggests mouse GLUT5 is composed of 501 amino acids, and has 69-88% amino acid identity with human, rat, and rabbit GLUT5. Expression of mouse GLUT5 cRNA in Xenopus laevis oocytes showed that GLUT5 mediated fructose transport, with a Kt of 13 mM. Northern blot studies detected GLUT5 mRNA expression in mouse small intestine, kidney, and testis, with transcript sizes of approximately 2.1, 2.1, and 2.8 kb, respectively. 5′Rapid Amplification of cDNA Ends (5′RACE) determined that the differences in transcript sizes occurred because GLUT5 possessed alternative transcriptional initiation sites in somatic and germ cells. In agreement with studies in rats and rabbits, mouse small intestinal GLUT5 mRNA expression levels were increased following exposure to a 65% fructose-enriched diet. In addition, developmental studies showed a significant increase in GLUT5 mRNA expression levels in adult mouse testis when compared to prepubertal mouse testis. To begin to identify the cis-acting domains responsible for GLUT5 expression characteristics, a 7.7-kb GLUT5 genomic fragment was isolated from a mouse λfix11 library and sequenced. The clone contained exons 1-4 and 5′ flanking regions. Moreover, caudal homeobox gene (CdxA), upstream stimulatory factor (USF), and sex-determining region of Y (SRY) binding sites were identified in the 5′ flanking region that may be responsible for GLUT5's expression characteristics: tissue distribution, sensitivity to dietary fructose in the small intestine, and developmental expression in the testis.
AB - Mouse GLUT5 cDNA and a 7.7-kb genomic fragment have been isolated and characterized. The cDNA sequence suggests mouse GLUT5 is composed of 501 amino acids, and has 69-88% amino acid identity with human, rat, and rabbit GLUT5. Expression of mouse GLUT5 cRNA in Xenopus laevis oocytes showed that GLUT5 mediated fructose transport, with a Kt of 13 mM. Northern blot studies detected GLUT5 mRNA expression in mouse small intestine, kidney, and testis, with transcript sizes of approximately 2.1, 2.1, and 2.8 kb, respectively. 5′Rapid Amplification of cDNA Ends (5′RACE) determined that the differences in transcript sizes occurred because GLUT5 possessed alternative transcriptional initiation sites in somatic and germ cells. In agreement with studies in rats and rabbits, mouse small intestinal GLUT5 mRNA expression levels were increased following exposure to a 65% fructose-enriched diet. In addition, developmental studies showed a significant increase in GLUT5 mRNA expression levels in adult mouse testis when compared to prepubertal mouse testis. To begin to identify the cis-acting domains responsible for GLUT5 expression characteristics, a 7.7-kb GLUT5 genomic fragment was isolated from a mouse λfix11 library and sequenced. The clone contained exons 1-4 and 5′ flanking regions. Moreover, caudal homeobox gene (CdxA), upstream stimulatory factor (USF), and sex-determining region of Y (SRY) binding sites were identified in the 5′ flanking region that may be responsible for GLUT5's expression characteristics: tissue distribution, sensitivity to dietary fructose in the small intestine, and developmental expression in the testis.
KW - 5′RACE
KW - Developmental
KW - Dietary
KW - Fructose transporter
KW - GLUT5
UR - http://www.scopus.com/inward/record.url?scp=0037036121&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(02)00284-1
DO - 10.1016/S0167-4781(02)00284-1
M3 - Article
C2 - 12031501
AN - SCOPUS:0037036121
SN - 0167-4781
VL - 1576
SP - 191
EP - 197
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 1-2
ER -