Chrysanthemum morifolium flower extract inhibits adipogenesis of 3t3-l1 cells via ampk/sirt1 pathway activation

Mak Soon Lee, Yangha Kim

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Chrysanthemum (Chrysanthemum morifolium Ramat) flowers (CF) are widely consumed as herbal tea in many countries, including China. The aim of the present study was to examine the anti-adipogenic effect of hot water extraction of CF (HCF) on 3T3-L1 cells and their underlying cellular mechanisms. HCF treatment inhibited lipid accumulation under conditions that did not show the toxicity of 3T3-L1 adipocytes. The activity of glycerol-3-phosphate dehydrogenase (GPDH), which plays an important role in glycerol lipid metabolism, was also reduced by HCF. Adipogenesis/lipogenesis-related mRNA expression levels of peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer-binding protein-α (CEBP-α), sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid-binding protein 4 (FABP4), acetyl-CoA carboxylase 1 (ACC1), and fatty acid synthase (FAS) were suppressed by HCF in a dose-dependent manner. Moreover, HCF increased activities of AMP-activated protein kinase (AMPK) and sirtuin 1 (SIRT1), involved in lipid metabolism. These findings suggest that HCF inhibits adipocyte lipid accumulation through suppression of adipogenesis/lipogenesis-related gene expression and activation of the AMPK/SIRT1 pathway. Therefore, it suggests that HCF may be used as a potentially beneficial plant material for preventing obesity.

Original languageEnglish
Article number2726
Pages (from-to)1-12
Number of pages12
JournalNutrients
Volume12
Issue number9
DOIs
StatePublished - Sep 2020

Bibliographical note

Funding Information:
Funding: This study was supported by the National Research Foundation of Korea (NRF) funded by the Korean Government (MSIT) (numbers 2012M3A9C4048761 and 2019R1A2C1002861).

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) funded by the Korean Government (MSIT) (numbers 2012M3A9C4048761 and 2019R1A2C1002861). Acknowledgments: We thank Amway Korea and Amway China for providing the UPLC analysis and HCF extract.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • AMPK
  • Adipogenesis
  • Chrysanthemum morifolium flower
  • SIRT1

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