Chrysanthemum morifolium and Its Bioactive Substance Enhanced the Sleep Quality in Rodent Models via Cl Channel Activation

Mijin Kim, Yu Jaung Kim, Hyang Woon Lee, Jae Chul Jung, Seikwan Oh

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Dried Chrysanthemum morifolium (Chry) flowers have been used in Korea as a traditional insomnia treatment. In this study, the sleep-promoting activity and improving sleep quality of Chry extract (ext) and its active substance linarin were analyzed by pentobarbital-induced sleep experiment in mice and electroencephalography (EEG), electromyogram (EMG) analysis in rats. In a dose-dependent manner, Chry ext and linarin promoted longer sleep duration in the pentobarbital-induced sleep test compared to pentobarbital-only groups at both hypnotic and subhypnotic doses. Chry ext administration also significantly improved sleep quality, as seen in the relative power of low-frequency (delta) waves when compared with the control group. Linarin increased Cl uptake in the SH-SY5Y human cell line and chloride influx was reduced by bicuculline. After administration of Chry ext, the hippocampus, frontal cortex, and hypothalamus from rodents were collected and blotted for glutamic acid decarboxylase (GAD)65/67 and gamma-aminobutyric acid (GABA)A receptors subunit expression levels. The expression of α1-subunits, β2-subunits, and GAD65/67 of the GABAA receptor was modulated in the rodent brain. In conclusion, Chry ext augments pentobarbital-induced sleep duration and enhances sleep quality in EEG waves. These effects might be due to the activation of the Cl channel.

Original languageEnglish
Article number1309
Pages (from-to)1309
Number of pages7
Issue number6
StatePublished - Mar 2023

Bibliographical note

Funding Information:
This research was funded by Korea Evaluation Institute of Industrial Technology (KEIT; 20008980).

Publisher Copyright:
© 2023 by the authors.


  • GABA receptor
  • electroencephalogram
  • linarin
  • pentobarbital
  • sleep


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