Chondrocyte 3D-culture in RGD-modified crosslinked hydrogel with temperature-controllable modulus

Hyesun Lee, Bo Gyu Choi, Hyo Jung Moon, Jiyeon Choi, Kwideok Park, Byeongmoon Jeong, Dong Keun Han

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Based on the thermosensitivity of Pluronic® F127 and the cytointeractability of Arg-Gly-Asp (RGD) sequences, RGD-modified F127 dimethacrylate (FM-RGD) hydrogel was investigated as a 3-dimensional culture matrix for chondrocytes. Chondrocytes were encapsulated in the hydrogel by radical copolymerization of FM-RGD and FM in the presence of cells. The FM-RGD hydrogel modulus could be modulated by temperature variation from 15 °C (1,300 Pa) to the culture temperature of 37 °C (8,900 Pa), at which the hydrogel provided a condrocyte-compatible microenvironment. Compared with the hydrogel prepared from F127 dimethacrylate (FM) without RGD, the RGD-modified hydrogel produced significant (p<0.01) improvements in cell proliferation, DNA production, and viability while allowing the chondrocytes to maintain their original spherical phenotypes.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalMacromolecular Research
Volume20
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • biomaterial
  • biomimetic
  • hydrogel
  • modulus
  • stimuli-sensitive polymers

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