Cholic acid attenuates ER stress-induced cell death in coxsackievirus-B3 infection

Jae Young Han, Hae In Jeong, Cheol Woo Park, Jisoo Yoon, Jaeyoung Ko, Sang Jip Nam, Byung Kwan Lim

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Coxsackievirus Type B3 (CVB3) is an enterovirus that belongs to the Picornaviridae and causes various diseases such as myocarditis and hand-foot-mouth disease. However, an effective antiviral drug is still not developed. In this study, we looked for potential inhibitors of CVB3 replication by examining the survival of CVB3-infected HeLa cells. We detected an antiviral effect by cholic acid and identified it as a candidate inhibitor of CVB3 replication. Cholic acid circulates in the liver and intestines, and it helps the digestion and absorption of lipids in the small intestine. HeLa cells were cultured in 12-well plates and treated with cholic acid (1 and 10 µg/ml) and 106 PFU/ml of CVB3. After 16 h post-infection, the cells were lysed and subjected to western blot analysis and RT-PCR. The production of the viral capsid protein VP1 was dramatically decreased, and translation initiation factor eIF4G1 cleavage was significantly inhibited by treatment with 10 µg/ml cholic acid. Moreover, cholic acid inhibited ERK signaling in CVB3-infected HeLa cells. RT-PCR showed that the amounts of the CVB3 RNA genome and mRNA for the ER stress-related transcription factor ATF4 were significantly reduced. These results showed that cholic acid strongly reduced ER stress and CVB3 proliferation. This compound can be developed as a safe natural therapeutic agent for enterovirus infections.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume28
Issue number1
DOIs
StatePublished - Jan 2018

Keywords

  • Cholic acid
  • Coxsackievirus B3
  • ER stress
  • Myocarditis
  • Proliferation

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