Chloroform extract of deer antler inhibits osteoclast differentiation and bone resorption

Yin Ji Li, Tae Hee Kim, Han Bok Kwak, Zang Hee Lee, Soo Young Lee, Gil Ja Jhon

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29 Scopus citations


It has been reported that deer antler extract has anti-bone resorptive activity in vivo. However, little is known about the cellular and molecular mechanism of this effect. In this study, we investigated the effects of deer antler extracts on osteoclast differentiation and bone-resorption in vitro. Chloroform extract (CE-C) of deer antler inhibited osteoclast differentiation in mouse bone marrow cultures stimulated by receptor activator of NF-κB ligand (RANKL) and macrophage-colony stimulating factor (M-CSF). CE-C suppressed the activation of extracellular signal-regulated kinase (ERK), protein kinase B (PKB/Akt) and inhibitor of kappa B (I-κB) by RANKL in osteoclast precursor cells. It also inhibited the bone resorptive activity of differentiated osteoclasts that was accompanied by disruption of actin rings and induction of the apoptosis. These results demonstrate deer antler extract may be a useful remedy for the treatment of bone-resorption diseases such as osteoporosis.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalJournal of Ethnopharmacology
Issue number2
StatePublished - 5 Sep 2007

Bibliographical note

Funding Information:
This study was supported by a grant (KRF-2002-015-CP0240) from the Korean Research Foundation and the International Mobile Telecommunications 2000 R&D Project (01-PJ11-PG9-01NT00-0044) from the Ministry of Information and Communication, Republic of Korea.


  • Bone resorption
  • Cervus elaphus
  • Osteoclast
  • Osteoporosis


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