Chitosan sponges as tissue engineering scaffolds for bone formation

Yang Jo Seol; Jue Yeon Lee; Yoon Jeong Park; Yong Moo Lee; Young -Ku; In Chul Rhyu; Seung Jin Lee; Soo Boo Han; Chong Pyoung Chung

doi:10.1023/b:bile.0000032962.79531.fd

Chitosan sponges as tissue engineering scaffolds for bone formation

Yang Jo Seol, Jue Yeon Lee, Yoon Jeong Park, Yong Moo Lee, Young -Ku, In Chul Rhyu, Seung Jin Lee, Soo Boo Han, Chong Pyoung Chung

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

292 Scopus citations

Abstract

Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 μm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 × 10⁶ (day 1), 11 × 10⁶ (day 28) and 12 × 10⁶ (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) μg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.

Original language	English
Pages (from-to)	1037-1041
Number of pages	5
Journal	Biotechnology Letters
Volume	26
Issue number	13
DOIs	https://doi.org/10.1023/b:bile.0000032962.79531.fd
State	Published - Jun 2004

Bibliographical note

Funding Information:
This work was supported by a grant from the Ministry of Science and Technology (MOST), #98-N1-02-01-A-13, Republic of Korea.

Keywords

bone formation
chitosan sponges
osteoblast
tissue engineering

Access to Document

10.1023/b:bile.0000032962.79531.fd

Cite this

@article{05dd0fa657be4d2d809d5ed7753a7784,

title = "Chitosan sponges as tissue engineering scaffolds for bone formation",

abstract = "Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 μm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 × 106 (day 1), 11 × 106 (day 28) and 12 × 106 (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) μg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.",

keywords = "bone formation, chitosan sponges, osteoblast, tissue engineering",

author = "Seol, {Yang Jo} and Lee, {Jue Yeon} and Park, {Yoon Jeong} and Lee, {Yong Moo} and Young -Ku and Rhyu, {In Chul} and Lee, {Seung Jin} and Han, {Soo Boo} and Chung, {Chong Pyoung}",

note = "Funding Information: This work was supported by a grant from the Ministry of Science and Technology (MOST), #98-N1-02-01-A-13, Republic of Korea.",

year = "2004",

month = jun,

doi = "10.1023/b:bile.0000032962.79531.fd",

language = "English",

volume = "26",

pages = "1037--1041",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "13",

}

TY - JOUR

T1 - Chitosan sponges as tissue engineering scaffolds for bone formation

AU - Seol, Yang Jo

AU - Lee, Jue Yeon

AU - Park, Yoon Jeong

AU - Lee, Yong Moo

AU - -Ku, Young

AU - Rhyu, In Chul

AU - Lee, Seung Jin

AU - Han, Soo Boo

AU - Chung, Chong Pyoung

N1 - Funding Information: This work was supported by a grant from the Ministry of Science and Technology (MOST), #98-N1-02-01-A-13, Republic of Korea.

PY - 2004/6

Y1 - 2004/6

N2 - Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 μm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 × 106 (day 1), 11 × 106 (day 28) and 12 × 106 (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) μg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.

AB - Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 μm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 × 106 (day 1), 11 × 106 (day 28) and 12 × 106 (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) μg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.

KW - bone formation

KW - chitosan sponges

KW - osteoblast

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=3042765720&partnerID=8YFLogxK

U2 - 10.1023/b:bile.0000032962.79531.fd

DO - 10.1023/b:bile.0000032962.79531.fd

M3 - Article

C2 - 15218375

AN - SCOPUS:3042765720

SN - 0141-5492

VL - 26

SP - 1037

EP - 1041

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 13

ER -

Chitosan sponges as tissue engineering scaffolds for bone formation

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this