Chitosan sponges as tissue engineering scaffolds for bone formation

Yang Jo Seol, Jue Yeon Lee, Yoon Jeong Park, Yong Moo Lee, Young -Ku, In Chul Rhyu, Seung Jin Lee, Soo Boo Han, Chong Pyoung Chung

Research output: Contribution to journalArticlepeer-review

261 Scopus citations


Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 μm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 × 106 (day 1), 11 × 106 (day 28) and 12 × 106 (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) μg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.

Original languageEnglish
Pages (from-to)1037-1041
Number of pages5
JournalBiotechnology Letters
Issue number13
StatePublished - Jun 2004


  • bone formation
  • chitosan sponges
  • osteoblast
  • tissue engineering


Dive into the research topics of 'Chitosan sponges as tissue engineering scaffolds for bone formation'. Together they form a unique fingerprint.

Cite this