Different from the canonical right-handed B-DNA, a left-handed Z-DNA forms an alternating syn- and anti-base conformations along the double-stranded helix under physiological conditions. Z-DNA structure plays a role in transcriptional regulation, chromatin remodeling, and genome stability. To understand the biological function of Z-DNA and map the genome-wide Z-DNA-forming sites (ZFSs), a ChIP-Seq strategy is applied, which is a combination of chromatin immunoprecipitation (ChIP) and high-throughput DNA sequencing analysis. Cross-linked chromatin is sheared and its fragments associated with Z-DNA-binding proteins are mapped onto the reference genome sequence. The global information of ZFSs positioning can provide a useful resource for better understanding of DNA structure-dependent biological mechanism.
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||11|
|State||Published - 2023|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea (NRF-2014M3C9A3064548, NRF-2017M3C9A6047625,
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Z-DNA-binding domain
- Z-DNA-forming site