Characterization of tnnc1 as a novel tumor suppressor of lung adenocarcinoma

Suyeon Kim, Jaewon Kim, Yeonjoo Jung, Yukyung Jun, Yeonhwa Jung, Hee Young Lee, Juhee Keum, Byung Jo Park, Jinseon Lee, Jhingook Kim, Sanghyuk Lee, Jaesang Kim

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

In this study, we describe a novel function of TNNC1 (Troponin C1, Slow Skeletal and Cardiac Type), a component of actin-bound troponin, as a tumor suppressor of lung adenocarcinoma (LUAD). First, the expression of TNNC1 was strongly down-regulated in cancer tissues compared to matched normal lung tissues, and down-regulation of TNNC1 was shown to be strongly correlated with increased mortality among LUAD patients. Interestingly, TNNC1 expression was enhanced by suppression of KRAS, and ectopic expression of TNNC1 in turn inhibited KRASG12D-mediated anchorage independent growth of NIH3T3 cells. Consistently, activation of KRAS pathway in LUAD patients was shown to be strongly correlated with down-regulation of TNNC1. In addition, ectopic expression of TNNC1 inhibited colony formation of multiple LUAD cell lines and induced DNA damage, cell cycle arrest and ultimately apoptosis. We further examined potential correlations between expression levels of TNNC1 and various clinical parameters and found that low-level expression is significantly associated with invasiveness of the tumor. Indeed, RNA interference-mediated down-regulation of TNNC1 led to significant enhancement of invasiveness in vitro. Collectively, our data indicate that TNNC1 has a novel function as a tumor suppressor and is targeted for down-regulation by KRAS pathway during the carcinogenesis of LUAD.

Original languageEnglish
Pages (from-to)619-631
Number of pages13
JournalMolecules and Cells
Volume43
Issue number7
StatePublished - 2020

Keywords

  • Invasion
  • KRAS
  • Lung adenocarcinoma
  • TNNC1
  • Tumor suppressor

Fingerprint

Dive into the research topics of 'Characterization of tnnc1 as a novel tumor suppressor of lung adenocarcinoma'. Together they form a unique fingerprint.

Cite this