Characterization of the murine gene encoding 1-Cys peroxiredoxin and identification of highly homologous genes

Tae Hoon Lee, Seong Lan Yu, Sun Uk Kim, Yong Man Kim, Inpyo Choi, Sang Won Kang, Sue Goo Rhee, Dae Yeul Yu

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


A new type of peroxiredoxin, named 1-Cys peroxiredoxin (1-Cys Prx), reduces hydrogen peroxide with the use of electrons from unidentified electron donor(s). We have isolated the mouse gene encoding 1-Cys Prx (CP-3) and shown that it is comprised of five exons and four introns. Analysis of 5' flanking regions revealed binding sequences of several putative transcription factors such as Sp1, Pit-1a, c-Jun, c-Myc and YY1. It is noticeable that several potential Sp1 binding sites assigned the -60 through -96 bp from putative transcription initiation site. The gel shift assays showed that Sp1 and Pit-1a bind specifically to each binding site in 1-Cys Prx promoter. We also isolated two highly related genes such as CP-2 and CP-5. These genes are encoded by single exons, and show 85% of nucleotide sequence homology with the CP-3. The structural features of these genes suggest that they might be intronless genes derived from the CP-3 by the mechanism involving retrotransposition. In addition, our data suggest that they are inserted to a specific site of the mouse L1 repetitive element. The 1-Cys Prx was actively transcribed in a variety of adult tissues as well as in the developing embryos. These results suggest that only the 1-Cys Prx gene might be relevant for studying the function of the 1-Cys Prx in the murine system. (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)337-344
Number of pages8
Issue number2
StatePublished - 8 Jul 1999

Bibliographical note

Funding Information:
This work was supported by a grant KM1243 from the Korea Ministry of Science and Technology.


  • Antioxidant enzyme
  • Bacterial artificial chromosome
  • Family gene
  • Retrotransposition


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