Characterization of polymorphic loci for two cicada species: Cryptotympana atrata and Hyalessa fuscata (Hemiptera: Cicadoidae)

The cicada species Cryptotympana atrata and Hyalessa fuscata are abundant in metropolitan Seoul where their population densities are positively correlated with local urban heat island intensities. Here, we characterized two panels of polymorphic markers for both species. A set of 21 microsatellite markers previously developed for a Chinese population of C. atrata were tested for the Korean population. Fifteen of the tested loci were found to be polymorphic in the target population. The number of alleles per locus ranged from 2 to 12, with observed and expected heterozygosities ranging from 0.2 to 1 and 0.06667 to 0.92644, respectively. Four of the tested loci were moderately polymorphic, and the rest were highly polymorphic. For H. fuscata, a novel panel of microsatellite markers was generated using a next-generation sequencing technique and 18 polymorphic loci were identified in the target population. The number of alleles per locus was between 3 and 16, with observed and expected heterozygosities ranging from 0.02222 to 0.97778 and 0.16804 to 0.86181, respectively. Polymorphism level was low in one locus, moderate in five loci, and high in the remaining loci. The microsatellite markers described in this study will be useful for identifying genetic structure and genetic differentiation among populations of C. atrata and H. fuscata in metropolitan Seoul.