Characterization of phenotypically distinct B-cell subsets and receptor-stimulated mitogen-activated protein kinase activation in human cord blood B cells

Yun Jung Ha, Yeung Chul Mun, Chu Myong Seong, Jong Ran Lee

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Human cord blood (CB) is a valuable source of hematopoietic stem cells, but clinical reports have indicated slow recovery of B-cell development and function after CB transplantation. To investigate the basis of these B-cell defects in reconstitution, we characterized B cells purified from CB. We compared B-cell receptor activation and B-cell subsets in CB, bone marrow (BM), and peripheral blood (PB). We found that in CB B cells activation of extracellular signal-regulated kinase (ERK) and p38 following ligation of CD40 but not of the B-cell antigen receptor (BCR) was inefficient. The patterns of expression of CD5, CD34, and CD40 in the B-cell population of CB were similar to those in PB rather than in BM. The B cells in CB contained an increased proportion of B cells expressing a high level of CD24 and a low proportion of B cells expressing CD27, pointing to the presence of circulating CD24 high immature transitional and CD27- naive B cells. CD40-mediated activation of ERK and p38 was also minimal in these B cells of CB. These findings may account for the functional defects of B cells in transplanted CB.

Original languageEnglish
Pages (from-to)1557-1564
Number of pages8
JournalJournal of Leukocyte Biology
Volume84
Issue number6
DOIs
StatePublished - 1 Dec 2008

Keywords

  • B-cell antigen receptor
  • CD40
  • Signal transduction
  • Transitional B cells

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