Characterization of nitrous oxide reduction by Azospira sp. HJ23 isolated from advanced wastewater treatment sludge

Hyung Joo Park; Ji Hyeon Kwon; Jeonghee Yun; Kyung Suk Cho

doi:10.1080/10934529.2020.1812321

Characterization of nitrous oxide reduction by Azospira sp. HJ23 isolated from advanced wastewater treatment sludge

Hyung Joo Park, Ji Hyeon Kwon, Jeonghee Yun, Kyung Suk Cho

Department of Environmental Science & Engineering

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18 Scopus citations

Abstract

A new nitrous oxide (N₂O)-reducing bacterium was isolated from a consortium that was enriched using advanced wastewater treatment sludge as an inoculum and N₂O as the sole nitrogen source. The isolated facultative anaerobe was identified as Azospira sp. HJ23. Azospira sp. HJ23 exhibited optimum N₂O-reducing activity with a C/N ratio of 62 at pH 6 in the temperature range of 37 °C to 40 °C. The optimum carbon source for N₂O reduction was a mixture of glucose and acetate. The maximum rate of N₂O reduction by Azospira sp. HJ23 was 4.8 mmol·g-dry cell⁻¹·h⁻¹, and its N₂O-reducing activity was higher than other known N₂O reducers. Azospira sp. HJ23 possessed several functional genes for denitrification. These included narG (NO₃^- reductase), nirK (NO₂^- reductase), norB (NO reductase), and nosZ (N₂O reductase) genes. These results suggest that Azospira sp. HJ23 can be applied in the denitrification process to minimalize N₂O emission.

Original language	English
Pages (from-to)	1459-1467
Number of pages	9
Journal	Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Volume	55
Issue number	12
DOIs	https://doi.org/10.1080/10934529.2020.1812321
State	Published - 2020

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2016R1A2B4015007).

Publisher Copyright:
© 2020 Taylor & Francis Group, LLC.

Keywords

Azospirasp
denitrification
functional denitrifying genes
greenhouse gas
Nitrous oxide

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10.1080/10934529.2020.1812321

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title = "Characterization of nitrous oxide reduction by Azospira sp. HJ23 isolated from advanced wastewater treatment sludge",

abstract = "A new nitrous oxide (N2O)-reducing bacterium was isolated from a consortium that was enriched using advanced wastewater treatment sludge as an inoculum and N2O as the sole nitrogen source. The isolated facultative anaerobe was identified as Azospira sp. HJ23. Azospira sp. HJ23 exhibited optimum N2O-reducing activity with a C/N ratio of 62 at pH 6 in the temperature range of 37 °C to 40 °C. The optimum carbon source for N2O reduction was a mixture of glucose and acetate. The maximum rate of N2O reduction by Azospira sp. HJ23 was 4.8 mmol·g-dry cell−1·h−1, and its N2O-reducing activity was higher than other known N2O reducers. Azospira sp. HJ23 possessed several functional genes for denitrification. These included narG (NO3- reductase), nirK (NO2- reductase), norB (NO reductase), and nosZ (N2O reductase) genes. These results suggest that Azospira sp. HJ23 can be applied in the denitrification process to minimalize N2O emission.",

keywords = "Azospirasp, denitrification, functional denitrifying genes, greenhouse gas, Nitrous oxide",

author = "Park, {Hyung Joo} and Kwon, {Ji Hyeon} and Jeonghee Yun and Cho, {Kyung Suk}",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2016R1A2B4015007). Publisher Copyright: {\textcopyright} 2020 Taylor & Francis Group, LLC.",

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Characterization of nitrous oxide reduction by Azospira sp. HJ23 isolated from advanced wastewater treatment sludge. / Park, Hyung Joo; Kwon, Ji Hyeon; Yun, Jeonghee et al.
In: Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, Vol. 55, No. 12, 2020, p. 1459-1467.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterization of nitrous oxide reduction by Azospira sp. HJ23 isolated from advanced wastewater treatment sludge

AU - Park, Hyung Joo

AU - Kwon, Ji Hyeon

AU - Yun, Jeonghee

AU - Cho, Kyung Suk

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2016R1A2B4015007). Publisher Copyright: © 2020 Taylor & Francis Group, LLC.

PY - 2020

Y1 - 2020

N2 - A new nitrous oxide (N2O)-reducing bacterium was isolated from a consortium that was enriched using advanced wastewater treatment sludge as an inoculum and N2O as the sole nitrogen source. The isolated facultative anaerobe was identified as Azospira sp. HJ23. Azospira sp. HJ23 exhibited optimum N2O-reducing activity with a C/N ratio of 62 at pH 6 in the temperature range of 37 °C to 40 °C. The optimum carbon source for N2O reduction was a mixture of glucose and acetate. The maximum rate of N2O reduction by Azospira sp. HJ23 was 4.8 mmol·g-dry cell−1·h−1, and its N2O-reducing activity was higher than other known N2O reducers. Azospira sp. HJ23 possessed several functional genes for denitrification. These included narG (NO3- reductase), nirK (NO2- reductase), norB (NO reductase), and nosZ (N2O reductase) genes. These results suggest that Azospira sp. HJ23 can be applied in the denitrification process to minimalize N2O emission.

AB - A new nitrous oxide (N2O)-reducing bacterium was isolated from a consortium that was enriched using advanced wastewater treatment sludge as an inoculum and N2O as the sole nitrogen source. The isolated facultative anaerobe was identified as Azospira sp. HJ23. Azospira sp. HJ23 exhibited optimum N2O-reducing activity with a C/N ratio of 62 at pH 6 in the temperature range of 37 °C to 40 °C. The optimum carbon source for N2O reduction was a mixture of glucose and acetate. The maximum rate of N2O reduction by Azospira sp. HJ23 was 4.8 mmol·g-dry cell−1·h−1, and its N2O-reducing activity was higher than other known N2O reducers. Azospira sp. HJ23 possessed several functional genes for denitrification. These included narG (NO3- reductase), nirK (NO2- reductase), norB (NO reductase), and nosZ (N2O reductase) genes. These results suggest that Azospira sp. HJ23 can be applied in the denitrification process to minimalize N2O emission.

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IS - 12

ER -

Characterization of nitrous oxide reduction by Azospira sp. HJ23 isolated from advanced wastewater treatment sludge

Abstract

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