TY - GEN
T1 - Characterization of cyclohexane and hexane degradation by Rhodococcus SP. EC1
AU - Lee, Eun Hee
AU - Cho, Kyung Suk
N1 - Funding Information:
This work was financially supported by the Korea Science and Engineering Foundation through the Advanced Environmental Biotechnology Research Center at Pohang University of Science and Technology (R11-2003-006-06001-0), and by the Korea Research Foundation (2006-512-D00037).
PY - 2007
Y1 - 2007
N2 - Cyclohexane is a recalcitrant compound that is more difficult to degrade than even n-alkanes or monoaromatic hydrocarbons. In this study, a cyclohexane-degrading consortium was obtained from oil-contaminated soil by an enrichment culture method. Based on a 16S rDNA PCR-DGGE (denaturing gradient gel electrophoresis) method, this consortium was identified as comprising alpha-proteobacteria, actionbacteria, and gamma-proteobacteria. One of these organisms, Rhodococcus sp. EC1, was isolated and shown to have excellent cyclohexane-degrading ability. The maximum specific cyclohexane degradation rate (Vmax) for EC1 was 246.4 umolg-DCW-1 (dry cell weight) -h-1. In addition to its cyclohexane degradation abilities, EC1 was also able to strongly degrade hexane, with a maximum specific hexane degradation rate of 361 μmol·g-DCW-1·h-1. Experiments using 14C-hexane revealed that EC1 mineralized 40.3% of hexane into CO2and converted 52.8% into biomass. Moreover, EC1 could use other hydrocarbons, including methanol, ethanol, acetone, methyl tert-butyl ether (MTBE), pyrene, diesel, lubricant oil, benzene, toluene, ethylbenzene, m-xylene, p-xylene and oxylene. These findings collectively suggest that EC1 may be a useful biological resource for removal of cyclohexane, hexane, and other recalcitrant hydrocarbons.
AB - Cyclohexane is a recalcitrant compound that is more difficult to degrade than even n-alkanes or monoaromatic hydrocarbons. In this study, a cyclohexane-degrading consortium was obtained from oil-contaminated soil by an enrichment culture method. Based on a 16S rDNA PCR-DGGE (denaturing gradient gel electrophoresis) method, this consortium was identified as comprising alpha-proteobacteria, actionbacteria, and gamma-proteobacteria. One of these organisms, Rhodococcus sp. EC1, was isolated and shown to have excellent cyclohexane-degrading ability. The maximum specific cyclohexane degradation rate (Vmax) for EC1 was 246.4 umolg-DCW-1 (dry cell weight) -h-1. In addition to its cyclohexane degradation abilities, EC1 was also able to strongly degrade hexane, with a maximum specific hexane degradation rate of 361 μmol·g-DCW-1·h-1. Experiments using 14C-hexane revealed that EC1 mineralized 40.3% of hexane into CO2and converted 52.8% into biomass. Moreover, EC1 could use other hydrocarbons, including methanol, ethanol, acetone, methyl tert-butyl ether (MTBE), pyrene, diesel, lubricant oil, benzene, toluene, ethylbenzene, m-xylene, p-xylene and oxylene. These findings collectively suggest that EC1 may be a useful biological resource for removal of cyclohexane, hexane, and other recalcitrant hydrocarbons.
UR - http://www.scopus.com/inward/record.url?scp=58449097868&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:58449097868
SN - 9781604239485
T3 - Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007
SP - 182
EP - 188
BT - Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007
PB - Battelle Press
T2 - 9th International In Situ and On-Site Bioremediation Symposium 2007
Y2 - 7 May 2007 through 10 May 2007
ER -