Characterization of cyclohexane and hexane degradation by Rhodococcus SP. EC1

Eun Hee Lee, Kyung Suk Cho

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Cyclohexane is a recalcitrant compound that is more difficult to degrade than even n-alkanes or monoaromatic hydrocarbons. In this study, a cyclohexane-degrading consortium was obtained from oil-contaminated soil by an enrichment culture method. Based on a 16S rDNA PCR-DGGE (denaturing gradient gel electrophoresis) method, this consortium was identified as comprising alpha-proteobacteria, actionbacteria, and gamma-proteobacteria. One of these organisms, Rhodococcus sp. EC1, was isolated and shown to have excellent cyclohexane-degrading ability. The maximum specific cyclohexane degradation rate (Vmax) for EC1 was 246.4 umolg-DCW-1 (dry cell weight) -h-1. In addition to its cyclohexane degradation abilities, EC1 was also able to strongly degrade hexane, with a maximum specific hexane degradation rate of 361 μmol·g-DCW-1·h-1. Experiments using 14C-hexane revealed that EC1 mineralized 40.3% of hexane into CO2and converted 52.8% into biomass. Moreover, EC1 could use other hydrocarbons, including methanol, ethanol, acetone, methyl tert-butyl ether (MTBE), pyrene, diesel, lubricant oil, benzene, toluene, ethylbenzene, m-xylene, p-xylene and oxylene. These findings collectively suggest that EC1 may be a useful biological resource for removal of cyclohexane, hexane, and other recalcitrant hydrocarbons.

Original languageEnglish
Title of host publicationBattelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007
Pages182-188
Number of pages7
StatePublished - 2007
Event9th International In Situ and On-Site Bioremediation Symposium 2007 - Baltimore, MD, United States
Duration: 7 May 200710 May 2007

Publication series

NameBattelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007
Volume1

Conference

Conference9th International In Situ and On-Site Bioremediation Symposium 2007
Country/TerritoryUnited States
CityBaltimore, MD
Period7/05/0710/05/07

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