Abstract
Cyclohexane is a recalcitrant compound that is more difficult to degrade than even n-alkanes or monoaromatic hydrocarbons. In this study, a cyclohexane-degrading consortium was obtained from oil-contaminated soil by an enrichment culture method. Based on a 16S rDNA PCR-DGGE (denaturing gradient gel electrophoresis) method, this consortium was identified as comprising alpha-proteobacteria, actionbacteria, and gamma-proteobacteria. One of these organisms, Rhodococcus sp. EC1, was isolated and shown to have excellent cyclohexane-degrading ability. The maximum specific cyclohexane degradation rate (Vmax) for EC1 was 246.4 umolg-DCW-1 (dry cell weight) -h-1. In addition to its cyclohexane degradation abilities, EC1 was also able to strongly degrade hexane, with a maximum specific hexane degradation rate of 361 μmol·g-DCW-1·h-1. Experiments using 14C-hexane revealed that EC1 mineralized 40.3% of hexane into CO2and converted 52.8% into biomass. Moreover, EC1 could use other hydrocarbons, including methanol, ethanol, acetone, methyl tert-butyl ether (MTBE), pyrene, diesel, lubricant oil, benzene, toluene, ethylbenzene, m-xylene, p-xylene and oxylene. These findings collectively suggest that EC1 may be a useful biological resource for removal of cyclohexane, hexane, and other recalcitrant hydrocarbons.
Original language | English |
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Title of host publication | Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007 |
Publisher | Battelle Press |
Pages | 182-188 |
Number of pages | 7 |
ISBN (Print) | 9781604239485 |
State | Published - 2007 |
Event | 9th International In Situ and On-Site Bioremediation Symposium 2007 - Baltimore, MD, United States Duration: 7 May 2007 → 10 May 2007 |
Publication series
Name | Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007 |
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Volume | 1 |
Conference
Conference | 9th International In Situ and On-Site Bioremediation Symposium 2007 |
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Country/Territory | United States |
City | Baltimore, MD |
Period | 7/05/07 → 10/05/07 |
Bibliographical note
Funding Information:This work was financially supported by the Korea Science and Engineering Foundation through the Advanced Environmental Biotechnology Research Center at Pohang University of Science and Technology (R11-2003-006-06001-0), and by the Korea Research Foundation (2006-512-D00037).