Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine

Jihyun Shin, Jeong Chan Joo, Eunji Lee, Sung Min Hyun, Hyun Joong Kim, Si Jae Park, Yung Hun Yang, Kyungmoon Park

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Cadaverine is used for the synthesis of the novel bio-polyamides 54, 56, and 510. Here, we examine the feasibility of using a lysine decarboxylase (LdcC) from Escherichia coli for high-level production of cadaverine. After sequential optimization of whole-cell biotransformation conditions, recombinant E. coli-overexpressing LdcC (EcLdcC) could produce 1.0 M cadaverine from 1.2 M crude l-lysine solution after 9 h. EcLdcC retained a higher cadaverine yield after being reused 10 times at acidic and alkaline pH values than that of a recombinant E. coli strain overexpressing an inducible lysine decarboxylase (CadA), a conventional cadaverine producer (90 vs. 51% at pH 6 and 55 vs. 15% at pH 8). This study reveals that EcLdcC is a promising whole-cell biocatalyst for the bio-based production of cadaverine from industrial grade l-lysine in comparison to EcCadA.

Original languageEnglish
Pages (from-to)909-924
Number of pages16
JournalApplied Biochemistry and Biotechnology
Volume185
Issue number4
DOIs
StatePublished - 1 Aug 2018

Keywords

  • Cadaverine
  • l-Lysine
  • Lysine decarboxylase
  • Reusability
  • Whole-cell biotransformation

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