Characterization of a nitrous oxide-reducing bacterial consortium

Hyung Joo Park; Ji Hyeon Kwon; Kyung Suk Cho

doi:10.4014/mbl.1908.08004

Characterization of a nitrous oxide-reducing bacterial consortium

Hyung Joo Park, Ji Hyeon Kwon, Kyung Suk Cho

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Nitrous oxide (N₂O) is a greenhouse gas with a global warming potential 310 times higher than that of carbon dioxide. In this study, an N₂O-reducing consortium was obtained by enrichment culture using advanced treatment sludge as the inoculum. The dominant bacteria in the consortium were Sulfurovum (17.95%), Geobacter (14.63%), Rectinema (11.45%), and Chlorobium (8.24%). The consortium displayed optimal N₂O reducing activity when acetate was supplied as the carbon source at a carbon/nitrogen ratio (mol·mol^-1) of 6.3. The N₂O reduction rate increased with increasing N₂O concentration at less than 3,000 ppm. Kinetic analysis revealed that the maximum N₂O reduction rate of the consortium was 163.9 µg-N·g-VSS^-1·h^-1. Genes present in the consortium included nosZ (reduction of nitrous oxide to N₂), narG (reduction of nitrate to nitrite), nirK (reduction of nitrite to nitric oxide), and norB (reduction of nitric oxide to nitrous oxide). These results indicate that the N₂O-reducing consortium is a promising bioresource that can be used in denitrification and N₂O mitigation.

Original language	English
Pages (from-to)	630-638
Number of pages	9
Journal	Korean Journal of Microbiology and Biotechnology
Volume	47
Issue number	4
DOIs	https://doi.org/10.4014/mbl.1908.08004
State	Published - 2019

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Science and ICT (2016R1A2B4015007).

Funding Information:
This paper was supported by the Semyung University Research Grant of 2017.

Publisher Copyright:
© 2019, The Korean Society for Microbiology and Biotechnology

Keywords

Consortium
Denitrification
Enrichment culture
Functional gene
Greenhouse gas
Nitrous oxide

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title = "Characterization of a nitrous oxide-reducing bacterial consortium",

abstract = "Nitrous oxide (N2O) is a greenhouse gas with a global warming potential 310 times higher than that of carbon dioxide. In this study, an N2O-reducing consortium was obtained by enrichment culture using advanced treatment sludge as the inoculum. The dominant bacteria in the consortium were Sulfurovum (17.95%), Geobacter (14.63%), Rectinema (11.45%), and Chlorobium (8.24%). The consortium displayed optimal N2O reducing activity when acetate was supplied as the carbon source at a carbon/nitrogen ratio (mol·mol-1) of 6.3. The N2O reduction rate increased with increasing N2O concentration at less than 3,000 ppm. Kinetic analysis revealed that the maximum N2O reduction rate of the consortium was 163.9 µg-N·g-VSS-1·h-1. Genes present in the consortium included nosZ (reduction of nitrous oxide to N2), narG (reduction of nitrate to nitrite), nirK (reduction of nitrite to nitric oxide), and norB (reduction of nitric oxide to nitrous oxide). These results indicate that the N2O-reducing consortium is a promising bioresource that can be used in denitrification and N2O mitigation.",

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author = "Park, {Hyung Joo} and Kwon, {Ji Hyeon} and Cho, {Kyung Suk}",

note = "Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Science and ICT (2016R1A2B4015007). Funding Information: This paper was supported by the Semyung University Research Grant of 2017. Publisher Copyright: {\textcopyright} 2019, The Korean Society for Microbiology and Biotechnology",

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AU - Kwon, Ji Hyeon

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N2 - Nitrous oxide (N2O) is a greenhouse gas with a global warming potential 310 times higher than that of carbon dioxide. In this study, an N2O-reducing consortium was obtained by enrichment culture using advanced treatment sludge as the inoculum. The dominant bacteria in the consortium were Sulfurovum (17.95%), Geobacter (14.63%), Rectinema (11.45%), and Chlorobium (8.24%). The consortium displayed optimal N2O reducing activity when acetate was supplied as the carbon source at a carbon/nitrogen ratio (mol·mol-1) of 6.3. The N2O reduction rate increased with increasing N2O concentration at less than 3,000 ppm. Kinetic analysis revealed that the maximum N2O reduction rate of the consortium was 163.9 µg-N·g-VSS-1·h-1. Genes present in the consortium included nosZ (reduction of nitrous oxide to N2), narG (reduction of nitrate to nitrite), nirK (reduction of nitrite to nitric oxide), and norB (reduction of nitric oxide to nitrous oxide). These results indicate that the N2O-reducing consortium is a promising bioresource that can be used in denitrification and N2O mitigation.

AB - Nitrous oxide (N2O) is a greenhouse gas with a global warming potential 310 times higher than that of carbon dioxide. In this study, an N2O-reducing consortium was obtained by enrichment culture using advanced treatment sludge as the inoculum. The dominant bacteria in the consortium were Sulfurovum (17.95%), Geobacter (14.63%), Rectinema (11.45%), and Chlorobium (8.24%). The consortium displayed optimal N2O reducing activity when acetate was supplied as the carbon source at a carbon/nitrogen ratio (mol·mol-1) of 6.3. The N2O reduction rate increased with increasing N2O concentration at less than 3,000 ppm. Kinetic analysis revealed that the maximum N2O reduction rate of the consortium was 163.9 µg-N·g-VSS-1·h-1. Genes present in the consortium included nosZ (reduction of nitrous oxide to N2), narG (reduction of nitrate to nitrite), nirK (reduction of nitrite to nitric oxide), and norB (reduction of nitric oxide to nitrous oxide). These results indicate that the N2O-reducing consortium is a promising bioresource that can be used in denitrification and N2O mitigation.

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Characterization of a nitrous oxide-reducing bacterial consortium

Abstract

Bibliographical note

Keywords

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