Characterization of a new ScbR-like γ-butyrolactone binding regulator (SlbR) in Streptomyces coelicolor

Yung Hun Yang, Eunjung Song, Ji Nu Kim, Bo Rahm Lee, Eun Jung Kim, Sung Hee Park, Woo Seong Kim, Hyung Yeon Park, Jong Min Jeon, Thangamani Rajesh, Yun Gon Kim, Byung Gee Kim

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

γ-Butyrolactones in Streptomyces are well recognized as bacterial hormones, and they affect secondary metabolism of Streptomyces. γ-Butyrolactone receptors are considered important regulatory proteins, and various γ-butyrolactone synthases and receptors have been reported in Streptomyces. Here, we characterized a new regulator, SCO0608, that interacted with SCB1 (γ-butyrolactone of Streptomyces coelicolor) and bound to the scbR/A and adpA promoters. The SCO0608 protein sequences are not similar to those of any known γ-butyrolactone binding proteins in Streptomyces such as ScbR from S. coelicolor or ArpA from Streptomyces griseus. Interestingly, SCO0608 functions as a repressor of antibiotic biosynthesis and spore formation in R5 complex media. We showed the existence of another type of γ-butyrolactone receptor in Streptomyces, and this SCO0608 was named ScbR-like γ-butyrolactone binding regulator (SlbR) in S. coelicolor.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume96
Issue number1
DOIs
StatePublished - Oct 2012

Keywords

  • γ-Butyrolactone
  • Antibiotic production
  • DNA affinity capture assay
  • ScbR
  • scbR/A promoter
  • Streptomyces coelicolor

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