Abstract
γ-Butyrolactones in Streptomyces are well recognized as bacterial hormones, and they affect secondary metabolism of Streptomyces. γ-Butyrolactone receptors are considered important regulatory proteins, and various γ-butyrolactone synthases and receptors have been reported in Streptomyces. Here, we characterized a new regulator, SCO0608, that interacted with SCB1 (γ-butyrolactone of Streptomyces coelicolor) and bound to the scbR/A and adpA promoters. The SCO0608 protein sequences are not similar to those of any known γ-butyrolactone binding proteins in Streptomyces such as ScbR from S. coelicolor or ArpA from Streptomyces griseus. Interestingly, SCO0608 functions as a repressor of antibiotic biosynthesis and spore formation in R5 complex media. We showed the existence of another type of γ-butyrolactone receptor in Streptomyces, and this SCO0608 was named ScbR-like γ-butyrolactone binding regulator (SlbR) in S. coelicolor.
Original language | English |
---|---|
Pages (from-to) | 113-121 |
Number of pages | 9 |
Journal | Applied Microbiology and Biotechnology |
Volume | 96 |
Issue number | 1 |
DOIs | |
State | Published - Oct 2012 |
Keywords
- γ-Butyrolactone
- Antibiotic production
- DNA affinity capture assay
- ScbR
- scbR/A promoter
- Streptomyces coelicolor