Characterization of a new ScbR-like γ-butyrolactone binding regulator (SlbR) in Streptomyces coelicolor

Yung Hun Yang; Eunjung Song; Ji Nu Kim; Bo Rahm Lee; Eun Jung Kim; Sung Hee Park; Woo Seong Kim; Hyung Yeon Park; Jong Min Jeon; Thangamani Rajesh; Yun Gon Kim; Byung Gee Kim

doi:10.1007/s00253-011-3803-4

Characterization of a new ScbR-like γ-butyrolactone binding regulator (SlbR) in Streptomyces coelicolor

Yung Hun Yang, Eunjung Song, Ji Nu Kim, Bo Rahm Lee, Eun Jung Kim, Sung Hee Park, Woo Seong Kim, Hyung Yeon Park, Jong Min Jeon, Thangamani Rajesh, Yun Gon Kim, Byung Gee Kim

Pharmaceutical Sciences

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17 Scopus citations

Abstract

γ-Butyrolactones in Streptomyces are well recognized as bacterial hormones, and they affect secondary metabolism of Streptomyces. γ-Butyrolactone receptors are considered important regulatory proteins, and various γ-butyrolactone synthases and receptors have been reported in Streptomyces. Here, we characterized a new regulator, SCO0608, that interacted with SCB1 (γ-butyrolactone of Streptomyces coelicolor) and bound to the scbR/A and adpA promoters. The SCO0608 protein sequences are not similar to those of any known γ-butyrolactone binding proteins in Streptomyces such as ScbR from S. coelicolor or ArpA from Streptomyces griseus. Interestingly, SCO0608 functions as a repressor of antibiotic biosynthesis and spore formation in R5 complex media. We showed the existence of another type of γ-butyrolactone receptor in Streptomyces, and this SCO0608 was named ScbR-like γ-butyrolactone binding regulator (SlbR) in S. coelicolor.

Original language	English
Pages (from-to)	113-121
Number of pages	9
Journal	Applied Microbiology and Biotechnology
Volume	96
Issue number	1
DOIs	https://doi.org/10.1007/s00253-011-3803-4
State	Published - Oct 2012

Keywords

γ-Butyrolactone
Antibiotic production
DNA affinity capture assay
ScbR
scbR/A promoter
Streptomyces coelicolor

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10.1007/s00253-011-3803-4

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title = "Characterization of a new ScbR-like γ-butyrolactone binding regulator (SlbR) in Streptomyces coelicolor",

abstract = "γ-Butyrolactones in Streptomyces are well recognized as bacterial hormones, and they affect secondary metabolism of Streptomyces. γ-Butyrolactone receptors are considered important regulatory proteins, and various γ-butyrolactone synthases and receptors have been reported in Streptomyces. Here, we characterized a new regulator, SCO0608, that interacted with SCB1 (γ-butyrolactone of Streptomyces coelicolor) and bound to the scbR/A and adpA promoters. The SCO0608 protein sequences are not similar to those of any known γ-butyrolactone binding proteins in Streptomyces such as ScbR from S. coelicolor or ArpA from Streptomyces griseus. Interestingly, SCO0608 functions as a repressor of antibiotic biosynthesis and spore formation in R5 complex media. We showed the existence of another type of γ-butyrolactone receptor in Streptomyces, and this SCO0608 was named ScbR-like γ-butyrolactone binding regulator (SlbR) in S. coelicolor.",

keywords = "γ-Butyrolactone, Antibiotic production, DNA affinity capture assay, ScbR, scbR/A promoter, Streptomyces coelicolor",

author = "Yang, {Yung Hun} and Eunjung Song and Kim, {Ji Nu} and Lee, {Bo Rahm} and Kim, {Eun Jung} and Park, {Sung Hee} and Kim, {Woo Seong} and Park, {Hyung Yeon} and Jeon, {Jong Min} and Thangamani Rajesh and Kim, {Yun Gon} and Kim, {Byung Gee}",

note = "Funding Information: Acknowledgments The authors thank John Innes Center for providing the cosmid library. This work was supported by the Global Frontier Program for Intelligent Synthetic Biology, Basic Science Research Program (2010-0009942) and Priority Research Centers Program (2009-0094021) through the National Research Foundation grant funded by the Korea Government (MEST). This subject is also partially supported by the Korea Ministry of Environment as a “Converging Technology Project (201-101-007).”",

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doi = "10.1007/s00253-011-3803-4",

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AU - Yang, Yung Hun

AU - Song, Eunjung

AU - Kim, Ji Nu

AU - Lee, Bo Rahm

AU - Kim, Eun Jung

AU - Park, Sung Hee

AU - Kim, Woo Seong

AU - Park, Hyung Yeon

AU - Jeon, Jong Min

AU - Rajesh, Thangamani

AU - Kim, Yun Gon

AU - Kim, Byung Gee

N1 - Funding Information: Acknowledgments The authors thank John Innes Center for providing the cosmid library. This work was supported by the Global Frontier Program for Intelligent Synthetic Biology, Basic Science Research Program (2010-0009942) and Priority Research Centers Program (2009-0094021) through the National Research Foundation grant funded by the Korea Government (MEST). This subject is also partially supported by the Korea Ministry of Environment as a “Converging Technology Project (201-101-007).”

PY - 2012/10

Y1 - 2012/10

N2 - γ-Butyrolactones in Streptomyces are well recognized as bacterial hormones, and they affect secondary metabolism of Streptomyces. γ-Butyrolactone receptors are considered important regulatory proteins, and various γ-butyrolactone synthases and receptors have been reported in Streptomyces. Here, we characterized a new regulator, SCO0608, that interacted with SCB1 (γ-butyrolactone of Streptomyces coelicolor) and bound to the scbR/A and adpA promoters. The SCO0608 protein sequences are not similar to those of any known γ-butyrolactone binding proteins in Streptomyces such as ScbR from S. coelicolor or ArpA from Streptomyces griseus. Interestingly, SCO0608 functions as a repressor of antibiotic biosynthesis and spore formation in R5 complex media. We showed the existence of another type of γ-butyrolactone receptor in Streptomyces, and this SCO0608 was named ScbR-like γ-butyrolactone binding regulator (SlbR) in S. coelicolor.

AB - γ-Butyrolactones in Streptomyces are well recognized as bacterial hormones, and they affect secondary metabolism of Streptomyces. γ-Butyrolactone receptors are considered important regulatory proteins, and various γ-butyrolactone synthases and receptors have been reported in Streptomyces. Here, we characterized a new regulator, SCO0608, that interacted with SCB1 (γ-butyrolactone of Streptomyces coelicolor) and bound to the scbR/A and adpA promoters. The SCO0608 protein sequences are not similar to those of any known γ-butyrolactone binding proteins in Streptomyces such as ScbR from S. coelicolor or ArpA from Streptomyces griseus. Interestingly, SCO0608 functions as a repressor of antibiotic biosynthesis and spore formation in R5 complex media. We showed the existence of another type of γ-butyrolactone receptor in Streptomyces, and this SCO0608 was named ScbR-like γ-butyrolactone binding regulator (SlbR) in S. coelicolor.

KW - γ-Butyrolactone

KW - Antibiotic production

KW - DNA affinity capture assay

KW - ScbR

KW - scbR/A promoter

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SN - 0175-7598

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JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 1

ER -

Characterization of a new ScbR-like γ-butyrolactone binding regulator (SlbR) in Streptomyces coelicolor

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Keywords

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