TY - JOUR
T1 - Characterization of 15 microsatellite loci and genetic analysis of Heterodera schachtii (Nematoda: Heteroderidae) in South Korea
AU - Kim, Jiyeon
AU - Kim, Taeho
AU - Lee, Yu Cheol
AU - Chun, Jae Yong
AU - Kern, Elizabeth M.A.
AU - Jung, Jongwoo
AU - Park, Joong Ki
N1 - Funding Information:
This research was supported by a research grant from the Animal and Plant Quarantine Agency (No. Z-1541785-2013-15-03 ); the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology ( NRF-2013R1A1A2005898 ); and grants from the Marine Biotechnology Program [Genome Analysis of Marine Organisms and Development of Functional Applications (PJT200620) and Marine Mollusk Resource Bank of Korea (20100053)] funded by the Ministry of Oceans and Fisheries of the Republic of Korea.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Heterodera schachtii, the sugar beet cyst nematode, is a major pest of agricultural crops worldwide. We report the development of fifteen polymorphic microsatellite markers and assess the genetic diversity and structure of three populations following a recent invasion of a previously unaffected region. Populations had low levels of heterozygosity, likely indicative of population structure, history, and inbreeding. Genetic diversity analysis suggested that the current infestation in South Korea may have come either from a single source population of mixed ancestry, or from multiple sources, indicating that implementing adequate prevention measures is still an unmet challenge. Much more work is needed on this species to identify global patterns of spread, and the microsatellite loci we develop here should be useful in many regions for modeling range expansion, studying the evolution of resistance, and increasing the effectiveness of pest management strategies.
AB - Heterodera schachtii, the sugar beet cyst nematode, is a major pest of agricultural crops worldwide. We report the development of fifteen polymorphic microsatellite markers and assess the genetic diversity and structure of three populations following a recent invasion of a previously unaffected region. Populations had low levels of heterozygosity, likely indicative of population structure, history, and inbreeding. Genetic diversity analysis suggested that the current infestation in South Korea may have come either from a single source population of mixed ancestry, or from multiple sources, indicating that implementing adequate prevention measures is still an unmet challenge. Much more work is needed on this species to identify global patterns of spread, and the microsatellite loci we develop here should be useful in many regions for modeling range expansion, studying the evolution of resistance, and increasing the effectiveness of pest management strategies.
KW - Genetic diversity
KW - Heterodera schachtii
KW - Microsatellite markers
KW - Simple sequence repeat (SSR)
KW - South Korea
KW - Sugar beet cyst nematode
UR - http://www.scopus.com/inward/record.url?scp=84949845676&partnerID=8YFLogxK
U2 - 10.1016/j.bse.2015.11.013
DO - 10.1016/j.bse.2015.11.013
M3 - Article
AN - SCOPUS:84949845676
SN - 0305-1978
VL - 64
SP - 97
EP - 104
JO - Biochemical Systematics and Ecology
JF - Biochemical Systematics and Ecology
ER -