Characterization of 15 microsatellite loci and genetic analysis of Heterodera schachtii (Nematoda: Heteroderidae) in South Korea

Jiyeon Kim; Taeho Kim; Yu Cheol Lee; Jae Yong Chun; Elizabeth M.A. Kern; Jongwoo Jung; Joong Ki Park

doi:10.1016/j.bse.2015.11.013

Characterization of 15 microsatellite loci and genetic analysis of Heterodera schachtii (Nematoda: Heteroderidae) in South Korea

Jiyeon Kim, Taeho Kim, Yu Cheol Lee, Jae Yong Chun, Elizabeth M.A. Kern, Jongwoo Jung, Joong Ki Park

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Heterodera schachtii, the sugar beet cyst nematode, is a major pest of agricultural crops worldwide. We report the development of fifteen polymorphic microsatellite markers and assess the genetic diversity and structure of three populations following a recent invasion of a previously unaffected region. Populations had low levels of heterozygosity, likely indicative of population structure, history, and inbreeding. Genetic diversity analysis suggested that the current infestation in South Korea may have come either from a single source population of mixed ancestry, or from multiple sources, indicating that implementing adequate prevention measures is still an unmet challenge. Much more work is needed on this species to identify global patterns of spread, and the microsatellite loci we develop here should be useful in many regions for modeling range expansion, studying the evolution of resistance, and increasing the effectiveness of pest management strategies.

Original language	English
Pages (from-to)	97-104
Number of pages	8
Journal	Biochemical Systematics and Ecology
Volume	64
DOIs	https://doi.org/10.1016/j.bse.2015.11.013
State	Published - 1 Feb 2016

Bibliographical note

Funding Information:
This research was supported by a research grant from the Animal and Plant Quarantine Agency (No. Z-1541785-2013-15-03 ); the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology ( NRF-2013R1A1A2005898 ); and grants from the Marine Biotechnology Program [Genome Analysis of Marine Organisms and Development of Functional Applications (PJT200620) and Marine Mollusk Resource Bank of Korea (20100053)] funded by the Ministry of Oceans and Fisheries of the Republic of Korea.

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

Genetic diversity
Heterodera schachtii
Microsatellite markers
Simple sequence repeat (SSR)
South Korea
Sugar beet cyst nematode

Access to Document

10.1016/j.bse.2015.11.013

Cite this

@article{af6d74c2b8b34130b2893d035d920a80,

title = "Characterization of 15 microsatellite loci and genetic analysis of Heterodera schachtii (Nematoda: Heteroderidae) in South Korea",

abstract = "Heterodera schachtii, the sugar beet cyst nematode, is a major pest of agricultural crops worldwide. We report the development of fifteen polymorphic microsatellite markers and assess the genetic diversity and structure of three populations following a recent invasion of a previously unaffected region. Populations had low levels of heterozygosity, likely indicative of population structure, history, and inbreeding. Genetic diversity analysis suggested that the current infestation in South Korea may have come either from a single source population of mixed ancestry, or from multiple sources, indicating that implementing adequate prevention measures is still an unmet challenge. Much more work is needed on this species to identify global patterns of spread, and the microsatellite loci we develop here should be useful in many regions for modeling range expansion, studying the evolution of resistance, and increasing the effectiveness of pest management strategies.",

keywords = "Genetic diversity, Heterodera schachtii, Microsatellite markers, Simple sequence repeat (SSR), South Korea, Sugar beet cyst nematode",

author = "Jiyeon Kim and Taeho Kim and Lee, {Yu Cheol} and Chun, {Jae Yong} and Kern, {Elizabeth M.A.} and Jongwoo Jung and Park, {Joong Ki}",

note = "Funding Information: This research was supported by a research grant from the Animal and Plant Quarantine Agency (No. Z-1541785-2013-15-03 ); the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology ( NRF-2013R1A1A2005898 ); and grants from the Marine Biotechnology Program [Genome Analysis of Marine Organisms and Development of Functional Applications (PJT200620) and Marine Mollusk Resource Bank of Korea (20100053)] funded by the Ministry of Oceans and Fisheries of the Republic of Korea. Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

year = "2016",

month = feb,

day = "1",

doi = "10.1016/j.bse.2015.11.013",

language = "English",

volume = "64",

pages = "97--104",

journal = "Biochemical Systematics and Ecology",

issn = "0305-1978",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Characterization of 15 microsatellite loci and genetic analysis of Heterodera schachtii (Nematoda: Heteroderidae) in South Korea

AU - Kim, Jiyeon

AU - Kim, Taeho

AU - Lee, Yu Cheol

AU - Chun, Jae Yong

AU - Kern, Elizabeth M.A.

AU - Jung, Jongwoo

AU - Park, Joong Ki

N1 - Funding Information: This research was supported by a research grant from the Animal and Plant Quarantine Agency (No. Z-1541785-2013-15-03 ); the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology ( NRF-2013R1A1A2005898 ); and grants from the Marine Biotechnology Program [Genome Analysis of Marine Organisms and Development of Functional Applications (PJT200620) and Marine Mollusk Resource Bank of Korea (20100053)] funded by the Ministry of Oceans and Fisheries of the Republic of Korea. Publisher Copyright: © 2015 Elsevier Ltd.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Heterodera schachtii, the sugar beet cyst nematode, is a major pest of agricultural crops worldwide. We report the development of fifteen polymorphic microsatellite markers and assess the genetic diversity and structure of three populations following a recent invasion of a previously unaffected region. Populations had low levels of heterozygosity, likely indicative of population structure, history, and inbreeding. Genetic diversity analysis suggested that the current infestation in South Korea may have come either from a single source population of mixed ancestry, or from multiple sources, indicating that implementing adequate prevention measures is still an unmet challenge. Much more work is needed on this species to identify global patterns of spread, and the microsatellite loci we develop here should be useful in many regions for modeling range expansion, studying the evolution of resistance, and increasing the effectiveness of pest management strategies.

AB - Heterodera schachtii, the sugar beet cyst nematode, is a major pest of agricultural crops worldwide. We report the development of fifteen polymorphic microsatellite markers and assess the genetic diversity and structure of three populations following a recent invasion of a previously unaffected region. Populations had low levels of heterozygosity, likely indicative of population structure, history, and inbreeding. Genetic diversity analysis suggested that the current infestation in South Korea may have come either from a single source population of mixed ancestry, or from multiple sources, indicating that implementing adequate prevention measures is still an unmet challenge. Much more work is needed on this species to identify global patterns of spread, and the microsatellite loci we develop here should be useful in many regions for modeling range expansion, studying the evolution of resistance, and increasing the effectiveness of pest management strategies.

KW - Genetic diversity

KW - Heterodera schachtii

KW - Microsatellite markers

KW - Simple sequence repeat (SSR)

KW - South Korea

KW - Sugar beet cyst nematode

UR - http://www.scopus.com/inward/record.url?scp=84949845676&partnerID=8YFLogxK

U2 - 10.1016/j.bse.2015.11.013

DO - 10.1016/j.bse.2015.11.013

M3 - Article

AN - SCOPUS:84949845676

SN - 0305-1978

VL - 64

SP - 97

EP - 104

JO - Biochemical Systematics and Ecology

JF - Biochemical Systematics and Ecology

ER -

Characterization of 15 microsatellite loci and genetic analysis of Heterodera schachtii (Nematoda: Heteroderidae) in South Korea

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this