Characterization and application of chemical-resistant polyurethane-based enzyme and whole cell compartments

Diana Uhrich, Hyun Young Jang, Jin Byung Park, Jan von Langermann

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

This study presents the preparation and physical-chemical characterization of chemical resistant polyurethane-based compartments for biocatalytic application. The artificial compartments were prepared from an emulsion of polymer precursor and an aqueous phase that includes a biocatalytic reaction system. After curing, highly dispersed aqueous domains were obtained, which still contain the entire biocatalytic reaction system and remain fixed in the solid polymer preparation. The tensile and compression behavior of the prepared polymeric material is not significantly affected by the incorporation and facilitates excellent stability against various organic solvents and acid solutions. Thereby, the compartments can be used not only for enantioselective alcohol-dehydrogenase catalyzed reduction but also for a whole cell catalyzed hydrolysis of esters. Moreover, the compartmented whole-cell system was considerably stable to allow multiple reuses without a noticeable loss of catalytic activity of the incorporated whole cell catalytic reaction system.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalJournal of Biotechnology
Volume289
DOIs
StatePublished - 10 Jan 2019

Keywords

  • Alcohol dehydrogenase
  • Biocatalysis
  • Esterase
  • Immobilization
  • Inclusion
  • Organic solvent

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