Abstract
Zanthoxylum piperitum (prickly ash) was grown as a suspension culture in Schenk and Hildebrandt medium supplemented with 50 g sucrose 1 -1 and 0.5 mg 2,4-dichlorophenoxyacetic acid 1 -1 for 21 days with elicitation by pectin added at day 15. Volatile compounds were extracted from the culture and 3-hydroxy-2-butanone was identified by GC-MS as the most abundant compound, followed by γ-butyrolactone and 2,3-butanedione. 2,3-Butanedione, ethyl 3-methylbutyrate, and ethyl 2-methylpropanoate were identified as the most intense aroma-active compounds and represented the characteristic aroma of the culture.
Original language | English |
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Pages (from-to) | 551-556 |
Number of pages | 6 |
Journal | Biotechnology Letters |
Volume | 24 |
Issue number | 7 |
DOIs | |
State | Published - 2002 |
Bibliographical note
Funding Information:This study was supported by the Korean Science and Engineering Foundation (KOSEF) through the Research Center for New Bio-Materials in Agriculture at Seoul National University. Authors are grateful for the graduate fellowship provided by the Korean Ministry of Education through the Brain Korea 21 Project.
Keywords
- 2,3-butanedione
- Aroma-active compounds
- Pectin elicitation
- Suspension culture
- Zanthoxylum piperitum