Changes in NMDAR2 subunit mRNA levels during pentobarbital tolerance/withdrawal in the rat brain: An in situ hybridization study

Choon Gon Jang; Seikwan Oh; Ing Kang Ho

doi:10.1023/A:1020746505854

Changes in NMDAR2 subunit mRNA levels during pentobarbital tolerance/withdrawal in the rat brain: An in situ hybridization study

Choon Gon Jang, Seikwan Oh, Ing Kang Ho

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Little is known about the functional modulation of NMDA receptor subunits at the molecular level. Therefore, a series of experiments were Conducted to elucidate more fully the role of NMDA receptor subtypes in pentobarbital tolerance and withdrawal. We investigated the influence of centrally administered pentobarbital on the regulation of mRNA levels of the family of NMDA receptor 2 (NR2) subtypes (NR2A, NR2B, and NR2C) by in situ hybridization histochemistry in rat brain. Animals were rendered tolerant by continuous intracerebroventricular (i.c.v.) infusion with pentobarbital (300 μg/10 μl/hr for 6 days) through pre-implanted cannulae connected to osmotic mini-pumps, and dependent, by abrupt withdrawal from pentobarbital. The NR2A subunit mRNA was increased in cortical areas in pentobarbital tolerant and withdrawal rats. In contrast, the NR2B mRNA was decreased in parietal cortex and hippocampus in both tolerance and withdrawal rats. The level of NR2C mRNA was increased in withdrawal rats, while there was no change in tolerant rats. These results indicate that continuous i.c.v. infusion with pentobarbital alters NR2 subunit mRNA expression in the rat brain, suggesting that NR2 subunits may play an important role in the development of tolerance to and withdrawal from pentobarbital.

Original language	English
Pages (from-to)	1371-1377
Number of pages	7
Journal	Neurochemical Research
Volume	23
Issue number	11
DOIs	https://doi.org/10.1023/A:1020746505854
State	Published - 1998

Bibliographical note

Funding Information:
The authors thank Dr. T. Ma for his technical assistance and Dr. Susan E. Wellman for her helpful review of the manuscript. This work was supported by a grant NIDA 04480.

Keywords

In situ hybridization
MRNA
NMDA receptor
Pentobarbital
Tolerance
Withdrawal

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title = "Changes in NMDAR2 subunit mRNA levels during pentobarbital tolerance/withdrawal in the rat brain: An in situ hybridization study",

abstract = "Little is known about the functional modulation of NMDA receptor subunits at the molecular level. Therefore, a series of experiments were Conducted to elucidate more fully the role of NMDA receptor subtypes in pentobarbital tolerance and withdrawal. We investigated the influence of centrally administered pentobarbital on the regulation of mRNA levels of the family of NMDA receptor 2 (NR2) subtypes (NR2A, NR2B, and NR2C) by in situ hybridization histochemistry in rat brain. Animals were rendered tolerant by continuous intracerebroventricular (i.c.v.) infusion with pentobarbital (300 μg/10 μl/hr for 6 days) through pre-implanted cannulae connected to osmotic mini-pumps, and dependent, by abrupt withdrawal from pentobarbital. The NR2A subunit mRNA was increased in cortical areas in pentobarbital tolerant and withdrawal rats. In contrast, the NR2B mRNA was decreased in parietal cortex and hippocampus in both tolerance and withdrawal rats. The level of NR2C mRNA was increased in withdrawal rats, while there was no change in tolerant rats. These results indicate that continuous i.c.v. infusion with pentobarbital alters NR2 subunit mRNA expression in the rat brain, suggesting that NR2 subunits may play an important role in the development of tolerance to and withdrawal from pentobarbital.",

keywords = "In situ hybridization, MRNA, NMDA receptor, Pentobarbital, Tolerance, Withdrawal",

author = "Jang, {Choon Gon} and Seikwan Oh and Ho, {Ing Kang}",

note = "Funding Information: The authors thank Dr. T. Ma for his technical assistance and Dr. Susan E. Wellman for her helpful review of the manuscript. This work was supported by a grant NIDA 04480.",

year = "1998",

doi = "10.1023/A:1020746505854",

language = "English",

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journal = "Neurochemical Research",

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T1 - Changes in NMDAR2 subunit mRNA levels during pentobarbital tolerance/withdrawal in the rat brain

T2 - An in situ hybridization study

AU - Jang, Choon Gon

AU - Oh, Seikwan

AU - Ho, Ing Kang

N1 - Funding Information: The authors thank Dr. T. Ma for his technical assistance and Dr. Susan E. Wellman for her helpful review of the manuscript. This work was supported by a grant NIDA 04480.

PY - 1998

Y1 - 1998

N2 - Little is known about the functional modulation of NMDA receptor subunits at the molecular level. Therefore, a series of experiments were Conducted to elucidate more fully the role of NMDA receptor subtypes in pentobarbital tolerance and withdrawal. We investigated the influence of centrally administered pentobarbital on the regulation of mRNA levels of the family of NMDA receptor 2 (NR2) subtypes (NR2A, NR2B, and NR2C) by in situ hybridization histochemistry in rat brain. Animals were rendered tolerant by continuous intracerebroventricular (i.c.v.) infusion with pentobarbital (300 μg/10 μl/hr for 6 days) through pre-implanted cannulae connected to osmotic mini-pumps, and dependent, by abrupt withdrawal from pentobarbital. The NR2A subunit mRNA was increased in cortical areas in pentobarbital tolerant and withdrawal rats. In contrast, the NR2B mRNA was decreased in parietal cortex and hippocampus in both tolerance and withdrawal rats. The level of NR2C mRNA was increased in withdrawal rats, while there was no change in tolerant rats. These results indicate that continuous i.c.v. infusion with pentobarbital alters NR2 subunit mRNA expression in the rat brain, suggesting that NR2 subunits may play an important role in the development of tolerance to and withdrawal from pentobarbital.

AB - Little is known about the functional modulation of NMDA receptor subunits at the molecular level. Therefore, a series of experiments were Conducted to elucidate more fully the role of NMDA receptor subtypes in pentobarbital tolerance and withdrawal. We investigated the influence of centrally administered pentobarbital on the regulation of mRNA levels of the family of NMDA receptor 2 (NR2) subtypes (NR2A, NR2B, and NR2C) by in situ hybridization histochemistry in rat brain. Animals were rendered tolerant by continuous intracerebroventricular (i.c.v.) infusion with pentobarbital (300 μg/10 μl/hr for 6 days) through pre-implanted cannulae connected to osmotic mini-pumps, and dependent, by abrupt withdrawal from pentobarbital. The NR2A subunit mRNA was increased in cortical areas in pentobarbital tolerant and withdrawal rats. In contrast, the NR2B mRNA was decreased in parietal cortex and hippocampus in both tolerance and withdrawal rats. The level of NR2C mRNA was increased in withdrawal rats, while there was no change in tolerant rats. These results indicate that continuous i.c.v. infusion with pentobarbital alters NR2 subunit mRNA expression in the rat brain, suggesting that NR2 subunits may play an important role in the development of tolerance to and withdrawal from pentobarbital.

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Changes in NMDAR2 subunit mRNA levels during pentobarbital tolerance/withdrawal in the rat brain: An in situ hybridization study

Abstract

Bibliographical note

Keywords

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