Change of voltage-gated potassium channel 1.7 expressions in monocrotaline-induced pulmonary arterial hypertension rat model

Hyeryon Lee, Kwan Chang Kim, Young Mi Hong

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Purpose: Abnormal potassium channels expression affects vessel function, including vascular tone and proliferation rate. Diverse potassium channels, including voltage-gated potassium (Kv) channels, are involved in pathological changes of pulmonary arterial hypertension (PAH). Since the role of the Kv1.7 channel in PAH has not been previously studied, we investigated whether Kv1.7 channel expression changes in the lung tissue of a monocrotaline (MCT)-induced PAH rat model and whether this change is influenced by the endothelin (ET)-1 and reactive oxygen species (ROS) pathways. Methods: Rats were separated into 2 groups: the control (C) group and the MCT (M) group (60 mg/kg MCT). A hemodynamic study was performed by catheterization into the external jugular vein to estimate the right ventricular pressure (RVP), and pathological changes in the lung tissue were investigated. Changes in protein and mRNA levels were confirmed by western blot and polymerase chain reaction analysis, respectively. Results: MCT caused increased RVP, medial wall thickening of the pulmonary arterioles, and increased expression level of ET-1, ET receptor A, and NADPH oxidase (NOX) 4 proteins. Decreased Kv1.7 channel expression was detected in the lung tissue. Inward-rectifier channel 6.1 expression in the lung tissue also increased. We confirmed that ET-1 increased NOX4 level and decreased glutathione peroxidase-1 level in pulmonary artery smooth muscle cells (PASMCs). ET-1 increased ROS level in PASMCs. Conclusion: Decreased Kv1.7 channel expression might be caused by the ET-1 and ROS pathways and contributes to MCT-induced PAH.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalKorean Journal of Pediatrics
Issue number9
StatePublished - Sep 2018

Bibliographical note

Funding Information:
The authors gratefully acknowledge the support provided by Basic Science Research Program through the National Research Foundation of Korea funded by Ministry of Education (NRF-2013 R1A1A3004619, and NRF-2017R1D1A1B03030831).

Publisher Copyright:
© 2018 by The Korean Pediatric Society.


  • Hypertension
  • Potassium channels
  • Pulmonary
  • Reactive oxygen species


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