Cellular Uptake Mechanism of TCTP-PTD in Human Lung Carcinoma Cells

Hyo Young Kim, Sabin Kim, Hae Jun Pyun, Jeehye Maeng, Kyunglim Lee

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We reported previously that human translationally controlled tumor protein (TCTP) contains, at its NH2-terminus, a protein transduction domain (PTD), which we called TCTP-PTD, with the amino acid sequence MIIYRDLISH. In this report we describe how TCTP-PTD penetrates A549 human lung cancer cell membranes and promotes protein internalization. Cellular uptake of fluorescent TCTP-PTD and a recombinant fusion protein consisting of TCTP-PTD and GFP (green fluorescent protein) was analyzed by confocal fluorescence microscopy and flow cytometry. Inhibitor assays using several agents that perturb the internalization process revealed that TCTP-PTD transduces the cells partly via lipid-raft/caveola-dependent endocytosis and partly by macropinocytosis in a dynamin/actin/microtubule-dependent pathway. To trace the pathway followed by the penetration of TCTP-PTD, the localization of PTDs was investigated in the lipid-raft, subcellular, and ER fractions. We found that, after entry, TCTP-PTD is localized in the cytoplasm and cytoskeleton, but not in the nucleus, and is transported into endoplasmic reticulum (ER). Expression levels of caveolin-1 in A549 and HeLa cells are different, and these differences appear to contribute to the sensitivity of TCTP-PTD uptake inhibition, against lipid-raft depleter, nystatin. This elucidation of the underlying mechanism of TCTP-PTD translocation may help the design of approaches that employ TCTP-PTD in the cellular delivery of bioactive molecules.

Original languageEnglish
Pages (from-to)194-203
Number of pages10
JournalMolecular Pharmaceutics
Volume12
Issue number1
DOIs
StatePublished - 5 Jan 2015

Keywords

  • endocytosis pathway
  • lipid-rafts/caveolae
  • mechanism
  • protein transduction domain (PTD)
  • subcellular fractionations
  • translationally controlled tumor protein (TCTP)

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