Cellular uptake and cytotoxicity of octahedral rhenium cluster complexes

Soo Jin Choi; Konstantin A. Brylev; Jing Zhe Xu; Yuri V. Mironov; Vladimir E. Fedorov; Youn Soo Sohn; Sung Jin Kim; Jin Ho Choy

doi:10.1016/j.jinorgbio.2008.07.013

Cellular uptake and cytotoxicity of octahedral rhenium cluster complexes

Soo Jin Choi, Konstantin A. Brylev, Jing Zhe Xu, Yuri V. Mironov, Vladimir E. Fedorov, Youn Soo Sohn, Sung Jin Kim, Jin Ho Choy

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Cellular uptake behavior of a novel class of octahedral rhenium cluster compounds, hexahydroxo complexes K₄[{Re₆S₈}(OH)₆] · 8H₂O (1) and K₄[{Re₆Se₈}(OH)₆] · 8H₂O (2), was evaluated in human cervical adenocarcinoma HeLa cells. Confocal microscopy and flow cytometry studies demonstrated that rhenium cluster 1 was not internalized into cell, while rhenium cluster 2 was. Conjugation of a polymer to rhenium cluster 1, namely the derivative K₄[{Re₆S₈}(OH)₅L] (3) (L is amphiphilic diblock copolymer MPEG550-CH₂CONH-GlyPheLeuGlyPheLeu-COO^-), considerably enhanced cellular uptake in a concentration-dependent manner and was predominantly localized in the cytoplasm and nucleus upon incubation time. The uptake of rhenium cluster 2 was mediated by energy-dependent endocytosis, whereas rhenium cluster 3 was directly ingested into cells by cell-fusion-like mechanism. According to the cytotoxicity evaluation test, both rhenium clusters 2 and 3 did not exhibit acute cytotoxic effects up to 50 μM, at the practical concentration level of biological applications. It is, therefore, expected that the rhenium cluster complexes can be promising potential candidates as diagnostic agents for medical treatment.

Original language	English
Pages (from-to)	1991-1996
Number of pages	6
Journal	Journal of Inorganic Biochemistry
Volume	102
Issue number	11
DOIs	https://doi.org/10.1016/j.jinorgbio.2008.07.013
State	Published - Nov 2008

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. R11-2005-008-00000-0), by the Korea Research Foundation Grant Funded by the Ministry of Education and Human Resource Development (Grant KRF-2005-210-C00015) and by Goskontrakt 02.513.11.3047/2007-3-1.3-07-01-093.

Keywords

Amphiphilic diblock copolymer
Cellular uptake
Cytotoxicity
Octahedral rhenium cluster complexes
Uptake mechanism

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10.1016/j.jinorgbio.2008.07.013

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title = "Cellular uptake and cytotoxicity of octahedral rhenium cluster complexes",

abstract = "Cellular uptake behavior of a novel class of octahedral rhenium cluster compounds, hexahydroxo complexes K4[{Re6S8}(OH)6] · 8H2O (1) and K4[{Re6Se8}(OH)6] · 8H2O (2), was evaluated in human cervical adenocarcinoma HeLa cells. Confocal microscopy and flow cytometry studies demonstrated that rhenium cluster 1 was not internalized into cell, while rhenium cluster 2 was. Conjugation of a polymer to rhenium cluster 1, namely the derivative K4[{Re6S8}(OH)5L] (3) (L is amphiphilic diblock copolymer MPEG550-CH2CONH-GlyPheLeuGlyPheLeu-COO-), considerably enhanced cellular uptake in a concentration-dependent manner and was predominantly localized in the cytoplasm and nucleus upon incubation time. The uptake of rhenium cluster 2 was mediated by energy-dependent endocytosis, whereas rhenium cluster 3 was directly ingested into cells by cell-fusion-like mechanism. According to the cytotoxicity evaluation test, both rhenium clusters 2 and 3 did not exhibit acute cytotoxic effects up to 50 μM, at the practical concentration level of biological applications. It is, therefore, expected that the rhenium cluster complexes can be promising potential candidates as diagnostic agents for medical treatment.",

keywords = "Amphiphilic diblock copolymer, Cellular uptake, Cytotoxicity, Octahedral rhenium cluster complexes, Uptake mechanism",

author = "Choi, {Soo Jin} and Brylev, {Konstantin A.} and Xu, {Jing Zhe} and Mironov, {Yuri V.} and Fedorov, {Vladimir E.} and Sohn, {Youn Soo} and Kim, {Sung Jin} and Choy, {Jin Ho}",

note = "Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. R11-2005-008-00000-0), by the Korea Research Foundation Grant Funded by the Ministry of Education and Human Resource Development (Grant KRF-2005-210-C00015) and by Goskontrakt 02.513.11.3047/2007-3-1.3-07-01-093.",

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AU - Brylev, Konstantin A.

AU - Xu, Jing Zhe

AU - Mironov, Yuri V.

AU - Fedorov, Vladimir E.

AU - Sohn, Youn Soo

AU - Kim, Sung Jin

AU - Choy, Jin Ho

N1 - Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. R11-2005-008-00000-0), by the Korea Research Foundation Grant Funded by the Ministry of Education and Human Resource Development (Grant KRF-2005-210-C00015) and by Goskontrakt 02.513.11.3047/2007-3-1.3-07-01-093.

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N2 - Cellular uptake behavior of a novel class of octahedral rhenium cluster compounds, hexahydroxo complexes K4[{Re6S8}(OH)6] · 8H2O (1) and K4[{Re6Se8}(OH)6] · 8H2O (2), was evaluated in human cervical adenocarcinoma HeLa cells. Confocal microscopy and flow cytometry studies demonstrated that rhenium cluster 1 was not internalized into cell, while rhenium cluster 2 was. Conjugation of a polymer to rhenium cluster 1, namely the derivative K4[{Re6S8}(OH)5L] (3) (L is amphiphilic diblock copolymer MPEG550-CH2CONH-GlyPheLeuGlyPheLeu-COO-), considerably enhanced cellular uptake in a concentration-dependent manner and was predominantly localized in the cytoplasm and nucleus upon incubation time. The uptake of rhenium cluster 2 was mediated by energy-dependent endocytosis, whereas rhenium cluster 3 was directly ingested into cells by cell-fusion-like mechanism. According to the cytotoxicity evaluation test, both rhenium clusters 2 and 3 did not exhibit acute cytotoxic effects up to 50 μM, at the practical concentration level of biological applications. It is, therefore, expected that the rhenium cluster complexes can be promising potential candidates as diagnostic agents for medical treatment.

AB - Cellular uptake behavior of a novel class of octahedral rhenium cluster compounds, hexahydroxo complexes K4[{Re6S8}(OH)6] · 8H2O (1) and K4[{Re6Se8}(OH)6] · 8H2O (2), was evaluated in human cervical adenocarcinoma HeLa cells. Confocal microscopy and flow cytometry studies demonstrated that rhenium cluster 1 was not internalized into cell, while rhenium cluster 2 was. Conjugation of a polymer to rhenium cluster 1, namely the derivative K4[{Re6S8}(OH)5L] (3) (L is amphiphilic diblock copolymer MPEG550-CH2CONH-GlyPheLeuGlyPheLeu-COO-), considerably enhanced cellular uptake in a concentration-dependent manner and was predominantly localized in the cytoplasm and nucleus upon incubation time. The uptake of rhenium cluster 2 was mediated by energy-dependent endocytosis, whereas rhenium cluster 3 was directly ingested into cells by cell-fusion-like mechanism. According to the cytotoxicity evaluation test, both rhenium clusters 2 and 3 did not exhibit acute cytotoxic effects up to 50 μM, at the practical concentration level of biological applications. It is, therefore, expected that the rhenium cluster complexes can be promising potential candidates as diagnostic agents for medical treatment.

KW - Amphiphilic diblock copolymer

KW - Cellular uptake

KW - Cytotoxicity

KW - Octahedral rhenium cluster complexes

KW - Uptake mechanism

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DO - 10.1016/j.jinorgbio.2008.07.013

M3 - Article

C2 - 18783832

AN - SCOPUS:53449090295

SN - 0162-0134

VL - 102

SP - 1991

EP - 1996

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

IS - 11

ER -

Cellular uptake and cytotoxicity of octahedral rhenium cluster complexes

Abstract

Bibliographical note

Keywords

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