Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent

Anna A. Krasilnikova; Anastasiya O. Solovieva; Kristina E. Trifonova; Konstantin A. Brylev; Anton A. Ivanov; Sung Jin Kim; Michael A. Shestopalov; Maria S. Fufaeva; Alexander M. Shestopalov; Yuri V. Mironov; Alexander F. Poveshchenko; Lidia V. Shestopalova

doi:10.1002/cmmi.1707

Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent

Anna A. Krasilnikova, Anastasiya O. Solovieva, Kristina E. Trifonova, Konstantin A. Brylev, Anton A. Ivanov, Sung Jin Kim, Michael A. Shestopalov, Maria S. Fufaeva, Alexander M. Shestopalov, Yuri V. Mironov, Alexander F. Poveshchenko, Lidia V. Shestopalova

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The octahedral cluster compound Na₂H₈[{Re₆Se₈}(P(C₂H₄CONH₂)(C₂H₄COO)₂)₆] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD₅₀) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex H_n[{Re₆Se₈}(P(C₂H₄CONH₂)(C₂H₄COO)₂)₆]^n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice.

Original language	English
Pages (from-to)	459-466
Number of pages	8
Journal	Contrast Media and Molecular Imaging
Volume	11
Issue number	6
DOIs	https://doi.org/10.1002/cmmi.1707
State	Published - 1 Nov 2016

Bibliographical note

Funding Information:
This research was supported by the Russian Science Foundation (Grant No 15-15-10006). M. A. Shestopalov is also grateful for the Grant of the President of Russia (Grant No MK 4054.2015.3) and K. A. Brylev thanks the Brain Pool Program of the Korean Federation of Science and Technology Societies (KOFST) funded by the Ministry of Science, ICT, and Future Planning (151-S-1-3-1190).

Publisher Copyright:
Copyright © 2016 John Wiley & Sons, Ltd.

Keywords

acute intravenous toxicity
cellular internalization
drug development
octahedral rhenium cluster complex
safety assessment
vascular contrast agent

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10.1002/cmmi.1707

Cite this

Krasilnikova, A. A., Solovieva, A. O., Trifonova, K. E., Brylev, K. A., Ivanov, A. A., Kim, S. J., Shestopalov, M. A., Fufaeva, M. S., Shestopalov, A. M., Mironov, Y. V., Poveshchenko, A. F., & Shestopalova, L. V. (2016). Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent. Contrast Media and Molecular Imaging, 11(6), 459-466. https://doi.org/10.1002/cmmi.1707

@article{ade5146475a6416893a14729fbb67d73,

title = "Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent",

abstract = "The octahedral cluster compound Na2H8[\{Re6Se8\}(P(C2H4CONH2)(C2H4COO)2)6] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD50) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex Hn[\{Re6Se8\}(P(C2H4CONH2)(C2H4COO)2)6]n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice.",

keywords = "acute intravenous toxicity, cellular internalization, drug development, octahedral rhenium cluster complex, safety assessment, vascular contrast agent",

author = "Krasilnikova, \{Anna A.\} and Solovieva, \{Anastasiya O.\} and Trifonova, \{Kristina E.\} and Brylev, \{Konstantin A.\} and Ivanov, \{Anton A.\} and Kim, \{Sung Jin\} and Shestopalov, \{Michael A.\} and Fufaeva, \{Maria S.\} and Shestopalov, \{Alexander M.\} and Mironov, \{Yuri V.\} and Poveshchenko, \{Alexander F.\} and Shestopalova, \{Lidia V.\}",

note = "Funding Information: This research was supported by the Russian Science Foundation (Grant No 15-15-10006). M. A. Shestopalov is also grateful for the Grant of the President of Russia (Grant No MK 4054.2015.3) and K. A. Brylev thanks the Brain Pool Program of the Korean Federation of Science and Technology Societies (KOFST) funded by the Ministry of Science, ICT, and Future Planning (151-S-1-3-1190). Publisher Copyright: Copyright {\textcopyright} 2016 John Wiley \& Sons, Ltd.",

year = "2016",

month = nov,

day = "1",

doi = "10.1002/cmmi.1707",

language = "English",

volume = "11",

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Krasilnikova, AA, Solovieva, AO, Trifonova, KE, Brylev, KA, Ivanov, AA, Kim, SJ, Shestopalov, MA, Fufaeva, MS, Shestopalov, AM, Mironov, YV, Poveshchenko, AF & Shestopalova, LV 2016, 'Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent', Contrast Media and Molecular Imaging, vol. 11, no. 6, pp. 459-466. https://doi.org/10.1002/cmmi.1707

Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent. / Krasilnikova, Anna A.; Solovieva, Anastasiya O.; Trifonova, Kristina E. et al.
In: Contrast Media and Molecular Imaging, Vol. 11, No. 6, 01.11.2016, p. 459-466.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent

AU - Krasilnikova, Anna A.

AU - Solovieva, Anastasiya O.

AU - Trifonova, Kristina E.

AU - Brylev, Konstantin A.

AU - Ivanov, Anton A.

AU - Kim, Sung Jin

AU - Shestopalov, Michael A.

AU - Fufaeva, Maria S.

AU - Shestopalov, Alexander M.

AU - Mironov, Yuri V.

AU - Poveshchenko, Alexander F.

AU - Shestopalova, Lidia V.

N1 - Funding Information: This research was supported by the Russian Science Foundation (Grant No 15-15-10006). M. A. Shestopalov is also grateful for the Grant of the President of Russia (Grant No MK 4054.2015.3) and K. A. Brylev thanks the Brain Pool Program of the Korean Federation of Science and Technology Societies (KOFST) funded by the Ministry of Science, ICT, and Future Planning (151-S-1-3-1190). Publisher Copyright: Copyright © 2016 John Wiley & Sons, Ltd.

PY - 2016/11/1

Y1 - 2016/11/1

N2 - The octahedral cluster compound Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD50) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex Hn[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6]n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice.

AB - The octahedral cluster compound Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD50) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex Hn[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6]n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice.

KW - acute intravenous toxicity

KW - cellular internalization

KW - drug development

KW - octahedral rhenium cluster complex

KW - safety assessment

KW - vascular contrast agent

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U2 - 10.1002/cmmi.1707

DO - 10.1002/cmmi.1707

M3 - Article

C2 - 27491502

AN - SCOPUS:84980349630

SN - 1555-4309

VL - 11

SP - 459

EP - 466

JO - Contrast Media and Molecular Imaging

JF - Contrast Media and Molecular Imaging

IS - 6

ER -

Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent

Abstract

Bibliographical note

Keywords

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