Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent

Anna A. Krasilnikova, Anastasiya O. Solovieva, Kristina E. Trifonova, Konstantin A. Brylev, Anton A. Ivanov, Sung Jin Kim, Michael A. Shestopalov, Maria S. Fufaeva, Alexander M. Shestopalov, Yuri V. Mironov, Alexander F. Poveshchenko, Lidia V. Shestopalova

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The octahedral cluster compound Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD50) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex Hn[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6]n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice.

Original languageEnglish
Pages (from-to)459-466
Number of pages8
JournalContrast Media and Molecular Imaging
Volume11
Issue number6
DOIs
StatePublished - 1 Nov 2016

Keywords

  • acute intravenous toxicity
  • cellular internalization
  • drug development
  • octahedral rhenium cluster complex
  • safety assessment
  • vascular contrast agent

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