TY - JOUR
T1 - Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X-ray contrast agent
AU - Krasilnikova, Anna A.
AU - Solovieva, Anastasiya O.
AU - Trifonova, Kristina E.
AU - Brylev, Konstantin A.
AU - Ivanov, Anton A.
AU - Kim, Sung Jin
AU - Shestopalov, Michael A.
AU - Fufaeva, Maria S.
AU - Shestopalov, Alexander M.
AU - Mironov, Yuri V.
AU - Poveshchenko, Alexander F.
AU - Shestopalova, Lidia V.
N1 - Funding Information:
This research was supported by the Russian Science Foundation (Grant No 15-15-10006). M. A. Shestopalov is also grateful for the Grant of the President of Russia (Grant No MK 4054.2015.3) and K. A. Brylev thanks the Brain Pool Program of the Korean Federation of Science and Technology Societies (KOFST) funded by the Ministry of Science, ICT, and Future Planning (151-S-1-3-1190).
Publisher Copyright:
Copyright © 2016 John Wiley & Sons, Ltd.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - The octahedral cluster compound Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD50) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex Hn[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6]n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice.
AB - The octahedral cluster compound Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD50) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex Hn[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6]n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice.
KW - acute intravenous toxicity
KW - cellular internalization
KW - drug development
KW - octahedral rhenium cluster complex
KW - safety assessment
KW - vascular contrast agent
UR - http://www.scopus.com/inward/record.url?scp=84980349630&partnerID=8YFLogxK
U2 - 10.1002/cmmi.1707
DO - 10.1002/cmmi.1707
M3 - Article
C2 - 27491502
AN - SCOPUS:84980349630
VL - 11
SP - 459
EP - 466
JO - Contrast Media and Molecular Imaging
JF - Contrast Media and Molecular Imaging
SN - 1555-4309
IS - 6
ER -