Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP

Ruoyu Zhong, Matthew Sullivan, Neil Upreti, Roy Chen, Agustin De Ganzó, Kaichun Yang, Shujie Yang, Ke Jin, Ye He, Ke Li, Jianping Xia, Zhiteng Ma, Luke P. Lee, Tania Konry, Tony Jun Huang

Research output: Contribution to journalArticlepeer-review


The study of molecular mechanisms at the single-cell level holds immense potential for enhancing immunotherapy and understanding neuroinflammation and neurodegenerative diseases by identifying previously concealed pathways within a diverse range of paired cells. However, existing single-cell pairing platforms have limitations in low pairing efficiency, complex manual operation procedures, and single-use functionality. Here, we report a multiparametric cellular immunity analysis by a modular acoustofluidic platform: CIAMAP. This platform enables users to efficiently sort and collect effector-target (i.e., NK92-K562) cell pairs and monitor the real-time dynamics of immunological response formation. Furthermore, we conducted transcriptional and protein expression analyses to evaluate the pathways that mediate effector cytotoxicity toward target cells, as well as the synergistic effect of doxorubicin on the cellular immune response. Our CIAMAP can provide promising building blocks for high-throughput quantitative single-cell level coculture to understand intercellular communication while also empowering immunotherapy by precision analysis of immunological synapses.

Original languageEnglish
Article numbereadj9964
JournalScience Advances
Issue number51
StatePublished - Dec 2023

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