CD30/TNF receptor-associated factor interaction: NF-κB activation and binding specificity

Soo Young Lee, Sang Yull Lee, Gokul Kandala, Mei Ling Liou, Hsiou Chi Liou, Yongwon Choi

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163 Scopus citations


CD30 is a member of the tumor necrosis factor (TNF) receptor superfamily. CD30 is expressed on normal activated lymphocytes, on several vitally transformed T- or B-cell lines and on neoplastic cells of Hodgkin's lymphoma. The interaction of CD30 with its ligand induces pleiotropic effects on cells resulting in proliferation, differentiation, or death. The CD30 cytoplasmic tail interacts with TNF receptor-associated factors (TRAFs), which have been shown to transduce signals mediated by TNF-R2 and CD40. We demonstrate here that TRAF2 also plays an important role in CD30-induced NF- κB activation. We also show that TRAF2-mediated activation of NF-KB plays a role in the activation of HIV transcription induced by CD30 cross-linking. Detailed site-directed mutagenesis of the CD30 cytoplasmic tail reveals that there are two independent binding sites for TRAF, each interacting with a different domain of TRAF. Furthermore, we localized the TRAF-C binding site in CD30 to a 5-7 amino acid stretch.

Original languageEnglish
Pages (from-to)9699-9703
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number18
StatePublished - 3 Sep 1996


  • TRAF-C domain binding motif


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