Cathepsin B Imaging to Predict Quality of Engineered Cartilage

Ji Young Yhee; Yong Jin Kim; Ju Hee Ryu; Hong Yeol Yoon; Hyeyoun Chang; Jae Hyung Park; Hyukjin Lee; Hyon Seok Jang; Unyong Jeong; Kwangmeyung Kim; Sun Woong Kang

doi:10.1002/mabi.201500215

Cathepsin B Imaging to Predict Quality of Engineered Cartilage

Ji Young Yhee, Yong Jin Kim, Ju Hee Ryu, Hong Yeol Yoon, Hyeyoun Chang, Jae Hyung Park, Hyukjin Lee, Hyon Seok Jang, Unyong Jeong, Kwangmeyung Kim, Sun Woong Kang

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during the chondrogenesis. The probe is synthesized with a CB-cleavable peptide linked to a near infrared fluorescence (NIRF) dye and a dark quencher, which can recover the NIRF signal in the presence of CB enzyme. The CB activities in two different sets of chondrocytes are comparatively analyzed. The chondrocytes with higher CB activity show more extensive area of chondrogenesis that CB molecular imaging directly reflects the chondrogenic potency of the cells. The CB probe is expected to provide a reliable prediction for the quality of engineered cartilage by visualizing the activity of the relevant enzyme in chondrocytes.

Original language	English
Pages (from-to)	1224-1232
Number of pages	9
Journal	Macromolecular Bioscience
Volume	15
Issue number	9
DOIs	https://doi.org/10.1002/mabi.201500215
State	Published - 1 Sep 2015

Bibliographical note

Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

cartilage tissue engineering
cathepsin B
diagnosis
molecular imaging
transplantation

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10.1002/mabi.201500215

Cite this

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title = "Cathepsin B Imaging to Predict Quality of Engineered Cartilage",

abstract = "Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during the chondrogenesis. The probe is synthesized with a CB-cleavable peptide linked to a near infrared fluorescence (NIRF) dye and a dark quencher, which can recover the NIRF signal in the presence of CB enzyme. The CB activities in two different sets of chondrocytes are comparatively analyzed. The chondrocytes with higher CB activity show more extensive area of chondrogenesis that CB molecular imaging directly reflects the chondrogenic potency of the cells. The CB probe is expected to provide a reliable prediction for the quality of engineered cartilage by visualizing the activity of the relevant enzyme in chondrocytes.",

keywords = "cartilage tissue engineering, cathepsin B, diagnosis, molecular imaging, transplantation",

author = "Yhee, {Ji Young} and Kim, {Yong Jin} and Ryu, {Ju Hee} and Yoon, {Hong Yeol} and Hyeyoun Chang and Park, {Jae Hyung} and Hyukjin Lee and Jang, {Hyon Seok} and Unyong Jeong and Kwangmeyung Kim and Kang, {Sun Woong}",

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doi = "10.1002/mabi.201500215",

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AU - Yhee, Ji Young

AU - Kim, Yong Jin

AU - Ryu, Ju Hee

AU - Yoon, Hong Yeol

AU - Chang, Hyeyoun

AU - Park, Jae Hyung

AU - Lee, Hyukjin

AU - Jang, Hyon Seok

AU - Jeong, Unyong

AU - Kim, Kwangmeyung

AU - Kang, Sun Woong

PY - 2015/9/1

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N2 - Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during the chondrogenesis. The probe is synthesized with a CB-cleavable peptide linked to a near infrared fluorescence (NIRF) dye and a dark quencher, which can recover the NIRF signal in the presence of CB enzyme. The CB activities in two different sets of chondrocytes are comparatively analyzed. The chondrocytes with higher CB activity show more extensive area of chondrogenesis that CB molecular imaging directly reflects the chondrogenic potency of the cells. The CB probe is expected to provide a reliable prediction for the quality of engineered cartilage by visualizing the activity of the relevant enzyme in chondrocytes.

AB - Cathepsin B (CB)-specific molecular imaging probe is applied to monitor the changes of CB expression in 3D cultured chondrocytes during the chondrogenesis. The probe is synthesized with a CB-cleavable peptide linked to a near infrared fluorescence (NIRF) dye and a dark quencher, which can recover the NIRF signal in the presence of CB enzyme. The CB activities in two different sets of chondrocytes are comparatively analyzed. The chondrocytes with higher CB activity show more extensive area of chondrogenesis that CB molecular imaging directly reflects the chondrogenic potency of the cells. The CB probe is expected to provide a reliable prediction for the quality of engineered cartilage by visualizing the activity of the relevant enzyme in chondrocytes.

KW - cartilage tissue engineering

KW - cathepsin B

KW - diagnosis

KW - molecular imaging

KW - transplantation

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JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

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Cathepsin B Imaging to Predict Quality of Engineered Cartilage

Abstract

Bibliographical note

Keywords

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