Capillary electrophoresis methods for microRNAs assays: A review

Eunmi Ban, Eun Joo Song

Research output: Contribution to journalReview articlepeer-review

17 Scopus citations

Abstract

MicroRNAs (miRNAs) are short noncoding RNAs that conduct important roles in many cellular processes such as development, proliferation, differentiation, and apoptosis. In particular, circulating miRNAs have been proposed as biomarkers for cancer, diabetes, cardiovascular disease, and other illnesses. Therefore, determination of miRNA expression levels in various biofluids is important for the investigation of biological processes in health and disease and for discovering their potential as new biomarkers and drug targets. Capillary electrophoresis (CE) is emerging as a useful analytical tool for analyzing miRNA because of its simple sample preparation steps and efficient resolution of a diverse size range of compounds. In particular, CE with laser-induced fluorescence detection is a promising and relatively rapidly developing tool with the potential to provide high sensitivity and specificity in the analysis of miRNAs. This paper covers a short overview of the recent developments and applications of CE systems in miRNA studies in biological and biomedical areas.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalAnalytica Chimica Acta
Volume852
DOIs
StatePublished - 10 Dec 2014

Keywords

  • Capillary electrophoresis
  • Laser-induced fluorescence
  • Microchip-based capillary electrophoresis
  • MicroRNA

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