Camphene attenuates skeletal muscle atrophy by regulating oxidative stress and lipid metabolism in rats

Suji Baek, Jisu Kim, Byung Seok Moon, Sun Mi Park, Da Eun Jung, Seo Young Kang, Sang Ju Lee, Seung Jun Oh, Seung Hae Kwon, Myung Hee Nam, Hye Ok Kim, Hai Jeon Yoon, Bom Sahn Kim, Kang Pa Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Sarcopenia-or cachexia-related muscle atrophy is due to imbalanced energy metabolism and oxidative stress-induced muscle dysfunction. Monoterpenes play biological and pharmacological reactive oxygen species (ROS) scavenging roles. Hence, we explored the effects of camphene, a bicyclic monoterpene, on skeletal muscle atrophy in vitro and in vivo. We treated L6 myoblast cells with camphene and then examined the ROS-related oxidative stress using Mito Tracker™ Red FM and anti-8-oxoguanine antibody staining. To investigate lipid metabolism, we performed real-time polymerase chain reactions, holotomographic microscopy, and respiratory gas analysis. Rat muscle atrophy in in vivo models was observed using18 F-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography and immunocytochemistry. Camphene reversed the aberrant cell size and muscle morphology of L6 myoblasts under starvation and in in vivo models. Camphene also attenuated E3 ubiquitin ligase muscle RING-finger protein-1, mitochondrial fission, and 8-oxoguanine nuclear expression in starved myotubes and hydrogen peroxide (H2 O2)-treated cells. Moreover, camphene significantly regulated lipid metabolism in H2 O2-treated cells and in vivo models. These findings suggest that camphene may potentially affect skeletal muscle atrophy by regulating oxidative stress and lipid metabolism.

Original languageEnglish
Article number3731
Pages (from-to)1-13
Number of pages13
JournalNutrients
Volume12
Issue number12
DOIs
StatePublished - Dec 2020

Keywords

  • Camphene
  • Lipid metabolism
  • Muscle atrophy
  • Oxidative stress
  • Sarcopenia

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