Ca-α1T, a fly T-type Ca 2+ channel, negatively modulates sleep

Kyunghwa Jeong, Soyoung Lee, Haengsoo Seo, Yangkyun Oh, Donghoon Jang, Joonho Choe, Daesoo Kim, Jung Ha Lee, Walton D. Jones

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Mammalian T-type Ca 2+ channels are encoded by three separate genes (Ca v 3.1, 3.2, 3.3). These channels are reported to be sleep stabilizers important in the generation of the delta rhythms of deep sleep, but controversy remains. The identification of precise physiological functions for the T-type channels has been hindered, at least in part, by the potential for compensation between the products of these three genes and a lack of specific pharmacological inhibitors. Invertebrates have only one T-type channel gene, but its functions are even less well-studied. We cloned Ca-α1T, the only Ca v 3 channel gene in Drosophila melanogaster, expressed it in Xenopus oocytes and HEK-293 cells, and confirmed it passes typical T-type currents. Voltage-clamp analysis revealed the biophysical properties of Ca-α1T show mixed similarity, sometimes falling closer to Ca v 3.1, sometimes to Ca v 3.2, and sometimes to Ca v 3.3. We found Ca-α1T is broadly expressed across the adult fly brain in a pattern vaguely reminiscent of mammalian T-type channels. In addition, flies lacking Ca-α1T show an abnormal increase in sleep duration most pronounced during subjective day under continuous dark conditions despite normal oscillations of the circadian clock. Thus, our study suggests invertebrate T-type Ca 2+ channels promote wakefulness rather than stabilizing sleep.

Original languageEnglish
Article number17893
JournalScientific Reports
StatePublished - 9 Dec 2015

Bibliographical note

Funding Information:
K.J. would like to thank Sujin Chae for helpful technical assistance. This work was supported by the Priority Research Centers Program of the National Research Foundation of the Republic of Korea (2012-0006690) to J.-H.L., by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Science, ICT and Future Planning (2011-0031955) to D.K., and by grants from the National Research Foundation of the Republic of Korea to D.K. (NRF-2011-0028772), J.C. (NRF-2015-021435) and W.D.J. (2013R1A1A2011339).


Dive into the research topics of 'Ca-α1T, a fly T-type Ca 2+ channel, negatively modulates sleep'. Together they form a unique fingerprint.

Cite this