Butyrate-based n-butanol production from an engineered Shewanella oneidensis MR-1

Jong Min Jeon, Hun Suk Song, Doo Geun Lee, Ju Won Hong, Yoon Gi Hong, Yu Mi Moon, Shashi Kant Bhatia, Jeong Jun Yoon, Wooseong Kim, Yung Hun Yang

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


n-Butanol is considered as the next-generation biofuel, because its physiochemical properties are very similar to fossil fuels and it could be produced by Clostridia under anaerobic culture. Due to the difficulties of strict anaerobic culture, a host which can be used with facultative environment was being searched for n-butanol production. As an alternative, Shewanella oneidensis MR-1, which is known as facultative bacteria, was selected as a host and studied. A plasmid containing adhE2 encoding alcohol dehydrogenase, various CoA transferases (ctfAB, atoAD, pct, and ACT), and acs encoding acetyl-CoA synthetase were introduced and examined to S. oneidensis MR-1 to produce n-butanol. As a result, ctfAB, acs, and adhE2 overexpression in S. oneidensis-pJM102 showed the highest n-butanol production in the presence of 2% of N-acetylglucosamine (NAG), 0.3% of butyrate, and 0.1 mM of IPTG for 96 h under microaerobic condition. When more NAG and butyrate were fed, n-butanol production was enhanced, producing up to 160 mg/L of n-butanol. When metal ions or extra electrons were added to S. oneidensis-pJM102 for n-butanol production, metal ion as electron acceptor or supply of extra electron showed no significant effect on n-butanol production. Overall, we made a newly engineered S. oneidensis that could utilize NAG and butyrate to produce n-butanol. It could be used in further microaerobic condition and electricity supply studies.

Original languageEnglish
Pages (from-to)1195-1204
Number of pages10
JournalBioprocess and Biosystems Engineering
Issue number8
StatePublished - 1 Aug 2018

Bibliographical note

Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.


  • Electron transfer
  • Shewanella oneidensis MR-1
  • n-Butanol


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