TY - JOUR
T1 - Butyrate-based n-butanol production from an engineered Shewanella oneidensis MR-1
AU - Jeon, Jong Min
AU - Song, Hun Suk
AU - Lee, Doo Geun
AU - Hong, Ju Won
AU - Hong, Yoon Gi
AU - Moon, Yu Mi
AU - Bhatia, Shashi Kant
AU - Yoon, Jeong Jun
AU - Kim, Wooseong
AU - Yang, Yung Hun
N1 - Funding Information:
Fig. 7 Effect of metal ion and extra electron supply on n-butanol production in S. oneidensis-pJM102. Seven different metal ions were used as electron acceptors for n-butanol production (1: Control; 2: MnCl2; 3: CuCl2; 4: CaCl2; 5: CoCl2; 6: ZnCl2; 7: FeSO4; MgSO4). All samples were cultured for 48 h. a Growth of S. oneidensis- Acknowledgements This study was also supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2015M1A5A1037196, NRF2016R1D1A1B03932301), Research Program to solve social issues of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017M3A9E4077234), and Korea Institute of Energy Technology Evaluation and Planning (KETEP), and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (No. 20163010092150). Consulting service from the Microbial Carbohydrate Resource Bank (MCRB, Seoul, Korea) was kindly appreciated.
Publisher Copyright:
© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - n-Butanol is considered as the next-generation biofuel, because its physiochemical properties are very similar to fossil fuels and it could be produced by Clostridia under anaerobic culture. Due to the difficulties of strict anaerobic culture, a host which can be used with facultative environment was being searched for n-butanol production. As an alternative, Shewanella oneidensis MR-1, which is known as facultative bacteria, was selected as a host and studied. A plasmid containing adhE2 encoding alcohol dehydrogenase, various CoA transferases (ctfAB, atoAD, pct, and ACT), and acs encoding acetyl-CoA synthetase were introduced and examined to S. oneidensis MR-1 to produce n-butanol. As a result, ctfAB, acs, and adhE2 overexpression in S. oneidensis-pJM102 showed the highest n-butanol production in the presence of 2% of N-acetylglucosamine (NAG), 0.3% of butyrate, and 0.1 mM of IPTG for 96 h under microaerobic condition. When more NAG and butyrate were fed, n-butanol production was enhanced, producing up to 160 mg/L of n-butanol. When metal ions or extra electrons were added to S. oneidensis-pJM102 for n-butanol production, metal ion as electron acceptor or supply of extra electron showed no significant effect on n-butanol production. Overall, we made a newly engineered S. oneidensis that could utilize NAG and butyrate to produce n-butanol. It could be used in further microaerobic condition and electricity supply studies.
AB - n-Butanol is considered as the next-generation biofuel, because its physiochemical properties are very similar to fossil fuels and it could be produced by Clostridia under anaerobic culture. Due to the difficulties of strict anaerobic culture, a host which can be used with facultative environment was being searched for n-butanol production. As an alternative, Shewanella oneidensis MR-1, which is known as facultative bacteria, was selected as a host and studied. A plasmid containing adhE2 encoding alcohol dehydrogenase, various CoA transferases (ctfAB, atoAD, pct, and ACT), and acs encoding acetyl-CoA synthetase were introduced and examined to S. oneidensis MR-1 to produce n-butanol. As a result, ctfAB, acs, and adhE2 overexpression in S. oneidensis-pJM102 showed the highest n-butanol production in the presence of 2% of N-acetylglucosamine (NAG), 0.3% of butyrate, and 0.1 mM of IPTG for 96 h under microaerobic condition. When more NAG and butyrate were fed, n-butanol production was enhanced, producing up to 160 mg/L of n-butanol. When metal ions or extra electrons were added to S. oneidensis-pJM102 for n-butanol production, metal ion as electron acceptor or supply of extra electron showed no significant effect on n-butanol production. Overall, we made a newly engineered S. oneidensis that could utilize NAG and butyrate to produce n-butanol. It could be used in further microaerobic condition and electricity supply studies.
KW - Electron transfer
KW - Shewanella oneidensis MR-1
KW - n-Butanol
UR - http://www.scopus.com/inward/record.url?scp=85046623547&partnerID=8YFLogxK
U2 - 10.1007/s00449-018-1948-6
DO - 10.1007/s00449-018-1948-6
M3 - Article
C2 - 29737409
AN - SCOPUS:85046623547
SN - 1615-7591
VL - 41
SP - 1195
EP - 1204
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
IS - 8
ER -