Bringing single-molecule spectroscopy to macromolecular protein complexes

Chirlmin Joo, Mohamed Fareh, V. Narry Kim

Research output: Contribution to journalReview articlepeer-review

20 Scopus citations

Abstract

Single-molecule fluorescence spectroscopy offers real-time, nanometer-resolution information. Over the past two decades, this emerging single-molecule technique has been rapidly adopted to investigate the structural dynamics and biological functions of proteins. Despite this remarkable achievement, single-molecule fluorescence techniques must be extended to macromolecular protein complexes that are physiologically more relevant for functional studies. In this review, we present recent major breakthroughs for investigating protein complexes within cell extracts using single-molecule fluorescence. We outline the challenges, future prospects and potential applications of these new single-molecule fluorescence techniques in biological and clinical research.

Original languageEnglish
Pages (from-to)30-37
Number of pages8
JournalTrends in Biochemical Sciences
Volume38
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • Cell lysate
  • Macromolecular complex
  • Protein
  • Single-molecule fluorescence
  • Single-molecule immunoprecipitation
  • Single-molecule pull-down

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