Brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing the NF-κB pathway in human dermal fibroblasts

Young Rae Lee; Eun Mi Noh; Ji Hye Han; Jeong Mi Kim; Jin Ki Hwang; Bo Mi Hwang; Eun Yong Chung; Byeong Soo Kim; Sung Ho Lee; Seung Jin Lee; Jong Suk Kim

doi:10.1016/j.ejphar.2011.10.016

Brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing the NF-κB pathway in human dermal fibroblasts

Young Rae Lee, Eun Mi Noh, Ji Hye Han, Jeong Mi Kim, Jin Ki Hwang, Bo Mi Hwang, Eun Yong Chung, Byeong Soo Kim, Sung Ho Lee, Seung Jin Lee, Jong Suk Kim

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Brazilin (7, 11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10 (6H)-tetrol), the major component of Caesalpinia sappan L., is a natural red pigment used for histological staining. Recent studies have shown that brazilin exhibits distinct biological effects, including anti-hepatotoxicity, antiplatelet activity, and anti-inflammatory activities. In the present study, we evaluated the effects of brazilin on MMP-1 and -3 expressions in human dermal fibroblasts exposed to ultraviolet B (UVB) irradiation. Brazilin showed protective effect on UVB-induced loss of cell viability of fibroblasts. Brazilin also blocked significantly UVB-induced Reactive Oxygen Species generation in fibroblasts. Brazilin inhibited UVB-induced MMP-1/3 expressions and secretions in a dose-dependent manner. Moreover, UVB-induced NF-κB activation was completely blocked by treatment with brazilin. These findings suggest that brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing of NF-κB activation in human dermal fibroblasts. Thus, brazilin might be used as a potential agent for treatment of UV-induced skin photoaging.

Original language	English
Pages (from-to)	80-86
Number of pages	7
Journal	European Journal of Pharmacology
Volume	674
Issue number	2-3
DOIs	https://doi.org/10.1016/j.ejphar.2011.10.016
State	Published - 14 Jan 2012

Bibliographical note

Funding Information:
This work was supported by a grant from the Ministry of Science & Technology (MoST)/Korea Science & Engineering Foundation (KOSEF) ( M10528010003-05N2801-00310 ), and by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MEST) ( No-2010-0001355 ), Republic of Korea.

Keywords

Brazilin
Matrix metalloproteinase
Nuclear factor-κB
Photoaging
Ultraviolet B

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10.1016/j.ejphar.2011.10.016

Cite this

Lee, Y. R., Noh, E. M., Han, J. H., Kim, J. M., Hwang, J. K., Hwang, B. M., Chung, E. Y., Kim, B. S., Lee, S. H., Lee, S. J., & Kim, J. S. (2012). Brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing the NF-κB pathway in human dermal fibroblasts. European Journal of Pharmacology, 674(2-3), 80-86. https://doi.org/10.1016/j.ejphar.2011.10.016

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title = "Brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing the NF-κB pathway in human dermal fibroblasts",

abstract = "Brazilin (7, 11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10 (6H)-tetrol), the major component of Caesalpinia sappan L., is a natural red pigment used for histological staining. Recent studies have shown that brazilin exhibits distinct biological effects, including anti-hepatotoxicity, antiplatelet activity, and anti-inflammatory activities. In the present study, we evaluated the effects of brazilin on MMP-1 and -3 expressions in human dermal fibroblasts exposed to ultraviolet B (UVB) irradiation. Brazilin showed protective effect on UVB-induced loss of cell viability of fibroblasts. Brazilin also blocked significantly UVB-induced Reactive Oxygen Species generation in fibroblasts. Brazilin inhibited UVB-induced MMP-1/3 expressions and secretions in a dose-dependent manner. Moreover, UVB-induced NF-κB activation was completely blocked by treatment with brazilin. These findings suggest that brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing of NF-κB activation in human dermal fibroblasts. Thus, brazilin might be used as a potential agent for treatment of UV-induced skin photoaging.",

keywords = "Brazilin, Matrix metalloproteinase, Nuclear factor-κB, Photoaging, Ultraviolet B",

author = "Lee, {Young Rae} and Noh, {Eun Mi} and Han, {Ji Hye} and Kim, {Jeong Mi} and Hwang, {Jin Ki} and Hwang, {Bo Mi} and Chung, {Eun Yong} and Kim, {Byeong Soo} and Lee, {Sung Ho} and Lee, {Seung Jin} and Kim, {Jong Suk}",

note = "Funding Information: This work was supported by a grant from the Ministry of Science & Technology (MoST)/Korea Science & Engineering Foundation (KOSEF) ( M10528010003-05N2801-00310 ), and by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MEST) ( No-2010-0001355 ), Republic of Korea. ",

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AU - Kim, Jeong Mi

AU - Hwang, Jin Ki

AU - Hwang, Bo Mi

AU - Chung, Eun Yong

AU - Kim, Byeong Soo

AU - Lee, Sung Ho

AU - Lee, Seung Jin

AU - Kim, Jong Suk

N1 - Funding Information: This work was supported by a grant from the Ministry of Science & Technology (MoST)/Korea Science & Engineering Foundation (KOSEF) ( M10528010003-05N2801-00310 ), and by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MEST) ( No-2010-0001355 ), Republic of Korea.

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N2 - Brazilin (7, 11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10 (6H)-tetrol), the major component of Caesalpinia sappan L., is a natural red pigment used for histological staining. Recent studies have shown that brazilin exhibits distinct biological effects, including anti-hepatotoxicity, antiplatelet activity, and anti-inflammatory activities. In the present study, we evaluated the effects of brazilin on MMP-1 and -3 expressions in human dermal fibroblasts exposed to ultraviolet B (UVB) irradiation. Brazilin showed protective effect on UVB-induced loss of cell viability of fibroblasts. Brazilin also blocked significantly UVB-induced Reactive Oxygen Species generation in fibroblasts. Brazilin inhibited UVB-induced MMP-1/3 expressions and secretions in a dose-dependent manner. Moreover, UVB-induced NF-κB activation was completely blocked by treatment with brazilin. These findings suggest that brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing of NF-κB activation in human dermal fibroblasts. Thus, brazilin might be used as a potential agent for treatment of UV-induced skin photoaging.

AB - Brazilin (7, 11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10 (6H)-tetrol), the major component of Caesalpinia sappan L., is a natural red pigment used for histological staining. Recent studies have shown that brazilin exhibits distinct biological effects, including anti-hepatotoxicity, antiplatelet activity, and anti-inflammatory activities. In the present study, we evaluated the effects of brazilin on MMP-1 and -3 expressions in human dermal fibroblasts exposed to ultraviolet B (UVB) irradiation. Brazilin showed protective effect on UVB-induced loss of cell viability of fibroblasts. Brazilin also blocked significantly UVB-induced Reactive Oxygen Species generation in fibroblasts. Brazilin inhibited UVB-induced MMP-1/3 expressions and secretions in a dose-dependent manner. Moreover, UVB-induced NF-κB activation was completely blocked by treatment with brazilin. These findings suggest that brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing of NF-κB activation in human dermal fibroblasts. Thus, brazilin might be used as a potential agent for treatment of UV-induced skin photoaging.

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KW - Matrix metalloproteinase

KW - Nuclear factor-κB

KW - Photoaging

KW - Ultraviolet B

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JF - European Journal of Pharmacology

IS - 2-3

ER -

Brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing the NF-κB pathway in human dermal fibroblasts

Abstract

Bibliographical note

Keywords

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