Black rice (Oryza sativa L.) extract attenuates hepatic steatosis in C57BL/6 J mice fed a high-fat diet via fatty acid oxidation

Hwan Hee Jang, Mi Young Park, Heon Woong Kim, Young Min Lee, Kyung A. Hwang, Jae Hak Park, Dong Sik Park, Oran Kwon

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95 Scopus citations


Background: Two major risk factors for the onset of fatty liver disease are excessive alcohol intake and obesity, the latter being associated with non-alcoholic fatty liver disease (NAFLD). The aim of this study was to examine the effects of black rice extract (BRE) on hepatic steatosis and insulin resistance in high-fat diet-fed mice, providing a model of NAFLD. Methods. Twenty-four mice were randomly divided into three groups (n = 8 in each group): normal fat diet (ND), high fat diet (HF), and high fat diet supplemented with 1% (w/w) BRE (HF +1% BRE). The experimental diets were fed for seven weeks. Results: A HF induced hepatic steatosis with significant increases in the serum levels of free fatty acids (FFAs), triglyceride (TG), total cholesterol (TC), and insulin. By contrast, supplementary BRE (10 g/kg of diet) included in the HF alleviated hepatic steatosis and significantly decreased serum TG and TC levels (p < 0.01 for both). Dietary BRE also increased expression of fatty acid metabolism-related genes, including carnitine palmitoyltransferase (CPT1A), acyl-CoA oxidase (ACO), cytochrome P450 (CYP4A10), and peroxisome proliferator activated receptor (PPAR)- (p < 0.05 for all). Conclusions: Dietary BRE supplementation improved serum lipid profiles and significantly enhanced mRNA expression levels of fatty acid metabolism-related genes, primarily via -oxidation and -oxidation in the liver. Taken together, these findings suggest that a BRE-supplemented diet could be useful in reducing the risks of hepatic steatosis and related disorders, including hyperlipidemia and hyperglycemia.

Original languageEnglish
Article number27
JournalNutrition and Metabolism
StatePublished - 2012

Bibliographical note

Funding Information:
This study was supported by research project of National Academy of Agricultural Science of Rural Development Administration, Republic of Korea (Project No. PJ008839).


  • Black rice
  • Cyanidin-3-glucoside
  • Fatty acid oxidation
  • Hepatic steatosis
  • High-fat diet


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