Black cohosh inhibits 17β-estradiol-induced cell proliferation of endometrial adenocarcinoma cells

So Yun Park, Hee Ja Kim, Sa Ra Lee, Youn Hee Choi, Kyungah Jeong, Hyewon Chung

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

This study was conducted to investigate the effect of black cohosh (BC) extract on the proliferation and apoptosis of Ishikawa cells. Ishikawa human endometrial adenocarcinoma cells were treated with or without BC (1, 5, 10 and 25 μM) and cell proliferation and cytotoxicity were measured by CCK-8 assays and flow cytometry analysis. Additionally, Ishikawa cells were treated with 17β-estradiol (E2), E2 + progesterone and E2 + BC (5 and 10 μM) and the effect of BC and progesterone on E2-induced cell proliferation was analyzed. BC decreased the proliferation of Ishikawa cells at a dose-dependent rate compared with the control group (p < 0.05). The proliferation of Ishikawa cells increased in the presence of E2, whereas the subsequent addition of progesterone or BC decreased proliferation to the level of the control group (p < 0.05). The inhibitory effect of BC on E2-induced cell proliferation was greater than the inhibitory effect of progesterone. In conclusion, BC induces apoptosis in Ishikawa cells and suppresses E2-induced cell proliferation in Ishikawa cells. BC could be considered a candidate co-treatment agent of estrogen-dependent tumors, especially those involving endometrial cells.

Original languageEnglish
Pages (from-to)840-843
Number of pages4
JournalGynecological Endocrinology
Volume32
Issue number10
DOIs
StatePublished - 2 Oct 2016

Bibliographical note

Publisher Copyright:
© 2016 Informa UK Limited, trading as Taylor & Francis Group.

Keywords

  • Endometrium
  • estrogen
  • menopause
  • uterus

Fingerprint

Dive into the research topics of 'Black cohosh inhibits 17β-estradiol-induced cell proliferation of endometrial adenocarcinoma cells'. Together they form a unique fingerprint.

Cite this