@article{174811496b4f42508a9d2198d98c722c,
title = "Bispecific anti-mPDGFRβ x cotinine scFv-Cκ-scFv fusion protein and cotinine-duocarmycin can form antibody-drug conjugate-like complexes that exert cytotoxicity against mPDGFRβ expressing cells",
abstract = "Antibody selection for antibody-drug conjugates (ADCs) has traditionally depended on its internalization into the target cell, although ADC efficacy also relies on recycling of the receptor-ADC complex, endo-lysosomal trafficking, and subsequent linker/antibody proteolysis. In this study, we observed that a bispecific anti-murine platelet-derived growth factor receptor beta (mPDGFRβ) x cotinine single-chain variable fragment (scFv)-kappa constant region (Cκ)-scFv fusion protein and cotinine-duocarmycin can form an ADC-like complex to induce cytotoxicity against mPDGFRβ expressing cells. Multiple anti-mPDGFRβ antibody candidates can be produced in this bispecific scFv-Cκ-scFv fusion protein format and tested for their ability to deliver cotinine-conjugated cytotoxic drugs, thus providing an improved approach for antibody selection in ADC development.",
keywords = "Antibody-drug conjugate, Bispecific antibody, Cotinine, Duocarmycin",
author = "Soohyun Kim and Hyori Kim and Jo, {Dong Hyun} and Kim, {Jeong Hun} and Kim, {Su Ree} and Dongmin Kang and Dobeen Hwang and Junho Chung",
note = "Funding Information: This work was supported by the National Research Foundation funded by the Korean government (MSIP) (grant no. NRF-2017M3A9C8032204 , NRF-2015R1D1A1A09057154 and NRF-2015M3A9E6028949 ). This research was also partially supported by the New Drug Development Center, Asan Medical Center (2017-758). Funding Information: This work was supported by the National Research Foundation funded by the Korean government (MSIP) (grant no. NRF-2017M3A9C8032204, NRF-2015R1D1A1A09057154 and NRF-2015M3A9E6028949). This research was also partially supported by the New Drug Development Center, Asan Medical Center (2017-758). Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2019",
month = feb,
day = "1",
doi = "10.1016/j.ymeth.2018.10.002",
language = "English",
volume = "154",
pages = "125--135",
journal = "Methods",
issn = "1046-2023",
publisher = "Academic Press Inc.",
}