Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst

Byeonghun Lee, Saebom Lee, Hyeonsoo Kim, Kijun Jeong, Jinbyung Park, Eunyeol Lee, Jinwon Lee

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Objective: To produce 10-ketostearic acid from oleic acid.

Conclusion: This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum.

Results: Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 °C with 5 g cells l−1 and 2.5 g oleic acid l−1. Under these conditions, the cells produced 1.96 g 10-ketostearic acid l−1 from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l−1 h−1.

Original languageEnglish
Pages (from-to)1101-1106
Number of pages6
JournalBiotechnology Letters
Volume37
Issue number5
DOIs
StatePublished - 1 May 2015

Bibliographical note

Funding Information:
This work was supported by the MOTIE/KEIT R&D Program (10044604, Bioproduction of long-chain dicarboxylic acids from fatty acids and lipids).

Publisher Copyright:
© 2015, Springer Science+Business Media Dordrecht.

Keywords

  • 10-Ketostearic acid
  • Biocatalysts
  • Biotransformation
  • Corynebacterium glutamicum
  • Oleate hydratase
  • Oleic acid
  • Secondary alcohol dehydrogenase

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