Abstract
Objective: To produce 10-ketostearic acid from oleic acid.
Conclusion: This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum.
Results: Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 °C with 5 g cells l−1 and 2.5 g oleic acid l−1. Under these conditions, the cells produced 1.96 g 10-ketostearic acid l−1 from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l−1 h−1.
Original language | English |
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Pages (from-to) | 1101-1106 |
Number of pages | 6 |
Journal | Biotechnology Letters |
Volume | 37 |
Issue number | 5 |
DOIs | |
State | Published - 1 May 2015 |
Bibliographical note
Funding Information:This work was supported by the MOTIE/KEIT R&D Program (10044604, Bioproduction of long-chain dicarboxylic acids from fatty acids and lipids).
Publisher Copyright:
© 2015, Springer Science+Business Media Dordrecht.
Keywords
- 10-Ketostearic acid
- Biocatalysts
- Biotransformation
- Corynebacterium glutamicum
- Oleate hydratase
- Oleic acid
- Secondary alcohol dehydrogenase