@article{b50dd8ee96dd4ec09bc685677d4a6486,
title = "Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains",
abstract = "Sucrose utilization has been established in Escherichia coli strains by expression of Mannheimia succiniciproducens β-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coli strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] from sucrose. When recombinant E. coli strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coli strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P(3HB-co-LA) from sucrose. Among the examined E. coli strains, recombinant E. coli XL1-Blue produced the highest contents of P(3HB) (53.60 ± 2.55 wt%) and P(3HB-co-LA) (29.44 ± 0.39 wt%). In the batch fermentations, recombinant E. coli XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt% P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a cost-efficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source.",
keywords = "Escherichia coli, Polyhydroxyalkanoates, Sucrose",
author = "Sohn, {Yu Jung} and Kim, {Hee Taek} and Baritugo, {Kei Anne} and Song, {Hye Min} and Ryu, {Mi Hee} and Kang, {Kyoung Hee} and Jo, {Seo Young} and Hoyong Kim and Kim, {You Jin} and Choi, {Jong il} and Park, {Su Kyeong} and Joo, {Jeong Chan} and Park, {Si Jae}",
note = "Funding Information: This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea (NRF-2015M1A2A2035810), the C1 Gas Refinery Program through the NRF funded by the MSIT (NRF-2015 M3D3A1A01064926) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2018R1D1A1B07049359). We would like to thank Prof. Sang Yup Lee in KAIST for fruitful discussion. Funding Information: This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea ( NRF-2015M1A2A2035810 ), the C1 Gas Refinery Program through the NRF funded by the MSIT ( NRF-2015 M3D3A1A01064926 ) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) ( NRF-2018R1D1A1B07049359 ). We would like to thank Prof. Sang Yup Lee in KAIST for fruitful discussion. Publisher Copyright: {\textcopyright} 2020",
year = "2020",
month = apr,
day = "15",
doi = "10.1016/j.ijbiomac.2020.01.254",
language = "English",
volume = "149",
pages = "593--599",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",
}