Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains

Yu Jung Sohn; Hee Taek Kim; Kei Anne Baritugo; Hye Min Song; Mi Hee Ryu; Kyoung Hee Kang; Seo Young Jo; Hoyong Kim; You Jin Kim; Jong il Choi; Su Kyeong Park; Jeong Chan Joo; Si Jae Park

doi:10.1016/j.ijbiomac.2020.01.254

Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains

Yu Jung Sohn, Hee Taek Kim, Kei Anne Baritugo, Hye Min Song, Mi Hee Ryu, Kyoung Hee Kang, Seo Young Jo, Hoyong Kim, You Jin Kim, Jong il Choi, Su Kyeong Park, Jeong Chan Joo, Si Jae Park

Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Sucrose utilization has been established in Escherichia coli strains by expression of Mannheimia succiniciproducens β-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coli strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] from sucrose. When recombinant E. coli strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coli strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P(3HB-co-LA) from sucrose. Among the examined E. coli strains, recombinant E. coli XL1-Blue produced the highest contents of P(3HB) (53.60 ± 2.55 wt%) and P(3HB-co-LA) (29.44 ± 0.39 wt%). In the batch fermentations, recombinant E. coli XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt% P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a cost-efficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source.

Original language	English
Pages (from-to)	593-599
Number of pages	7
Journal	International Journal of Biological Macromolecules
Volume	149
DOIs	https://doi.org/10.1016/j.ijbiomac.2020.01.254
State	Published - 15 Apr 2020

Bibliographical note

Funding Information:
This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea (NRF-2015M1A2A2035810), the C1 Gas Refinery Program through the NRF funded by the MSIT (NRF-2015 M3D3A1A01064926) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2018R1D1A1B07049359). We would like to thank Prof. Sang Yup Lee in KAIST for fruitful discussion.

Funding Information:
This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea ( NRF-2015M1A2A2035810 ), the C1 Gas Refinery Program through the NRF funded by the MSIT ( NRF-2015 M3D3A1A01064926 ) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) ( NRF-2018R1D1A1B07049359 ). We would like to thank Prof. Sang Yup Lee in KAIST for fruitful discussion.

Publisher Copyright:
© 2020

Keywords

Escherichia coli
Polyhydroxyalkanoates
Sucrose

Access to Document

10.1016/j.ijbiomac.2020.01.254

Cite this

Sohn, Y. J., Kim, H. T., Baritugo, K. A., Song, H. M., Ryu, M. H., Kang, K. H., Jo, S. Y., Kim, H., Kim, Y. J., Choi, J. I., Park, S. K., Joo, J. C., & Park, S. J. (2020). Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains. International Journal of Biological Macromolecules, 149, 593-599. https://doi.org/10.1016/j.ijbiomac.2020.01.254

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title = "Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains",

abstract = "Sucrose utilization has been established in Escherichia coli strains by expression of Mannheimia succiniciproducens β-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coli strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] from sucrose. When recombinant E. coli strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coli strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P(3HB-co-LA) from sucrose. Among the examined E. coli strains, recombinant E. coli XL1-Blue produced the highest contents of P(3HB) (53.60 ± 2.55 wt%) and P(3HB-co-LA) (29.44 ± 0.39 wt%). In the batch fermentations, recombinant E. coli XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt% P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a cost-efficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source.",

keywords = "Escherichia coli, Polyhydroxyalkanoates, Sucrose",

author = "Sohn, {Yu Jung} and Kim, {Hee Taek} and Baritugo, {Kei Anne} and Song, {Hye Min} and Ryu, {Mi Hee} and Kang, {Kyoung Hee} and Jo, {Seo Young} and Hoyong Kim and Kim, {You Jin} and Choi, {Jong il} and Park, {Su Kyeong} and Joo, {Jeong Chan} and Park, {Si Jae}",

note = "Funding Information: This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea (NRF-2015M1A2A2035810), the C1 Gas Refinery Program through the NRF funded by the MSIT (NRF-2015 M3D3A1A01064926) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2018R1D1A1B07049359). We would like to thank Prof. Sang Yup Lee in KAIST for fruitful discussion. Funding Information: This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea ( NRF-2015M1A2A2035810 ), the C1 Gas Refinery Program through the NRF funded by the MSIT ( NRF-2015 M3D3A1A01064926 ) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) ( NRF-2018R1D1A1B07049359 ). We would like to thank Prof. Sang Yup Lee in KAIST for fruitful discussion. Publisher Copyright: {\textcopyright} 2020",

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doi = "10.1016/j.ijbiomac.2020.01.254",

language = "English",

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T1 - Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains

AU - Sohn, Yu Jung

AU - Kim, Hee Taek

AU - Baritugo, Kei Anne

AU - Song, Hye Min

AU - Ryu, Mi Hee

AU - Kang, Kyoung Hee

AU - Jo, Seo Young

AU - Kim, Hoyong

AU - Kim, You Jin

AU - Choi, Jong il

AU - Park, Su Kyeong

AU - Joo, Jeong Chan

AU - Park, Si Jae

N1 - Funding Information: This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea (NRF-2015M1A2A2035810), the C1 Gas Refinery Program through the NRF funded by the MSIT (NRF-2015 M3D3A1A01064926) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2018R1D1A1B07049359). We would like to thank Prof. Sang Yup Lee in KAIST for fruitful discussion. Funding Information: This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea ( NRF-2015M1A2A2035810 ), the C1 Gas Refinery Program through the NRF funded by the MSIT ( NRF-2015 M3D3A1A01064926 ) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) ( NRF-2018R1D1A1B07049359 ). We would like to thank Prof. Sang Yup Lee in KAIST for fruitful discussion. Publisher Copyright: © 2020

PY - 2020/4/15

Y1 - 2020/4/15

N2 - Sucrose utilization has been established in Escherichia coli strains by expression of Mannheimia succiniciproducens β-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coli strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] from sucrose. When recombinant E. coli strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coli strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P(3HB-co-LA) from sucrose. Among the examined E. coli strains, recombinant E. coli XL1-Blue produced the highest contents of P(3HB) (53.60 ± 2.55 wt%) and P(3HB-co-LA) (29.44 ± 0.39 wt%). In the batch fermentations, recombinant E. coli XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt% P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a cost-efficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source.

AB - Sucrose utilization has been established in Escherichia coli strains by expression of Mannheimia succiniciproducens β-fructofuranosidase (SacC), which hydrolyzes sucrose into glucose and fructose. Recombinant E. coli strains that can utilize sucrose were examined for their abilities to produce poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] from sucrose. When recombinant E. coli strains expressing Ralstonia eutropha PhaCAB and SacC were cultured in MR medium containing 20 g/L of sucrose, all recombinant E. coli strains could produce P(3HB) from sucrose. Also, recombinant E. coli strains expressing Pseudomonas sp. MBEL 6-19 PhaC1437, Clostridium propionicum Pct540, R. eutropha PhaAB enzymes along with SacC could produce P(3HB-co-LA) from sucrose. Among the examined E. coli strains, recombinant E. coli XL1-Blue produced the highest contents of P(3HB) (53.60 ± 2.55 wt%) and P(3HB-co-LA) (29.44 ± 0.39 wt%). In the batch fermentations, recombinant E. coli XL1-Blue strains completely consumed 20 g/L of sucrose as the sole carbon source and supported the production of 3.76 g/L of P(3HB) and 1.82 g/L of P(3HB-co-LA) with 38.21 wt% P(3HB) and 20.88 wt% P(3HB-co-LA) contents, respectively. Recombinant E. coli strains developed in this study can be used to establish a cost-efficient biorefinery for the production of polyhydroxyalkanoates (PHAs) from sucrose, which is an abundant and inexpensive carbon source.

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ER -

Biosynthesis of polyhydroxyalkanoates from sucrose by metabolically engineered Escherichia coli strains

Abstract

Bibliographical note

Keywords

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