Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains

Si Jae Park; Woo Suk Ahn; Phillip R. Green; Sang Yup Lee

doi:10.1002/bit.1097

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains

Si Jae Park
, Woo Suk Ahn
, Phillip R. Green
, Sang Yup Lee

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.

Original language	English
Pages (from-to)	81-86
Number of pages	6
Journal	Biotechnology and Bioengineering
Volume	74
Issue number	1
DOIs	https://doi.org/10.1002/bit.1097
State	Published - 5 Jul 2001

Keywords

Escherichia coli
Poly(3-hydroxybutyrate-co-3- hydroxyvalerate-co-3-hydroxyhexanoate)
Polyhydroxyalkanoates
Terpolyester

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title = "Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains",

abstract = "The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.",

keywords = "Escherichia coli, Poly(3-hydroxybutyrate-co-3- hydroxyvalerate-co-3-hydroxyhexanoate), Polyhydroxyalkanoates, Terpolyester",

author = "Park, \{Si Jae\} and Ahn, \{Woo Suk\} and Green, \{Phillip R.\} and Lee, \{Sang Yup\}",

year = "2001",

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doi = "10.1002/bit.1097",

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volume = "74",

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journal = "Biotechnology and Bioengineering",

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AU - Park, Si Jae

AU - Ahn, Woo Suk

AU - Green, Phillip R.

AU - Lee, Sang Yup

PY - 2001/7/5

Y1 - 2001/7/5

N2 - The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.

AB - The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.

KW - Escherichia coli

KW - Poly(3-hydroxybutyrate-co-3- hydroxyvalerate-co-3-hydroxyhexanoate)

KW - Polyhydroxyalkanoates

KW - Terpolyester

UR - https://www.scopus.com/pages/publications/0035811881

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DO - 10.1002/bit.1097

M3 - Article

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AN - SCOPUS:0035811881

SN - 0006-3592

VL - 74

SP - 81

EP - 86

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

IS - 1

ER -

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains

Abstract

Keywords

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