Abstract
The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.
Original language | English |
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Pages (from-to) | 81-86 |
Number of pages | 6 |
Journal | Biotechnology and Bioengineering |
Volume | 74 |
Issue number | 1 |
DOIs | |
State | Published - 5 Jul 2001 |
Keywords
- Escherichia coli
- Poly(3-hydroxybutyrate-co-3- hydroxyvalerate-co-3-hydroxyhexanoate)
- Polyhydroxyalkanoates
- Terpolyester