Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains

Si Jae Park, Woo Suk Ahn, Phillip R. Green, Sang Yup Lee

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalBiotechnology and Bioengineering
Volume74
Issue number1
DOIs
StatePublished - 5 Jul 2001

Keywords

  • Escherichia coli
  • Poly(3-hydroxybutyrate-co-3- hydroxyvalerate-co-3-hydroxyhexanoate)
  • Polyhydroxyalkanoates
  • Terpolyester

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