The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.
|Number of pages||6|
|Journal||Biotechnology and Bioengineering|
|State||Published - 5 Jul 2001|
- Escherichia coli
- Poly(3-hydroxybutyrate-co-3- hydroxyvalerate-co-3-hydroxyhexanoate)