Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains

Si Jae Park; Woo Suk Ahn; Phillip R. Green; Sang Yup Lee

doi:10.1002/bit.1097

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains

Si Jae Park, Woo Suk Ahn, Phillip R. Green, Sang Yup Lee

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.

Original language	English
Pages (from-to)	81-86
Number of pages	6
Journal	Biotechnology and Bioengineering
Volume	74
Issue number	1
DOIs	https://doi.org/10.1002/bit.1097
State	Published - 5 Jul 2001

Keywords

Escherichia coli
Poly(3-hydroxybutyrate-co-3- hydroxyvalerate-co-3-hydroxyhexanoate)
Polyhydroxyalkanoates
Terpolyester

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title = "Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains",

abstract = "The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.",

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year = "2001",

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AU - Green, Phillip R.

AU - Lee, Sang Yup

PY - 2001/7/5

Y1 - 2001/7/5

N2 - The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.

AB - The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxy-hexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer.

KW - Escherichia coli

KW - Poly(3-hydroxybutyrate-co-3- hydroxyvalerate-co-3-hydroxyhexanoate)

KW - Polyhydroxyalkanoates

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Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains

Abstract

Keywords

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