Biosynthesis of poly(2-hydroxybutyrate-co-lactate) in metabolically engineered Escherichia coli

Cheol Gi Chae, You Jin Kim, Se Jin Lee, Young Hoon Oh, Jung Eun Yang, Jeong Chan Joo, Kyoung Hee Kang, Young Ah Jang, Hyuk Lee, A. Reum Park, Bong Keun Song, Sang Yup Lee, Si Jae Park

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

We have previously reported in vivo biosynthesis of polyhydroxyalkanoates containing 2-hydroxyacid monomers such as lactate and 2-hydroxybutyrate in recombinant Escherichia coli strains by the expression of evolved Clostridium propionicum propionyl-CoA transferase (PctCp) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1Ps6-19). Here, we report the biosynthesis of poly(2-hydroxybutyrate-co-lactate)[P(2HB-co-LA)] by direct fermentation of metabolically engineered E. coli strain. Among E. coli strains WL3110, XL1-Blue, and BL21(DE3), recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 produced P(76.4mol%2HB-co-23.6mol%LA) to the highest content of 88 wt% when it was cultured in a chemically defined medium containing 20 g/L of glucose and 2 g/L of sodium 2-hydroxybutyrate. When recombinant E. coli XL1-Blue strain expressing PhaC1437 and Pct540 was cultured in a chemically defined medium containing 20 g/L of glucose and varying concentration of sodium 2-hydroxybutyrate, 2HB monomer fraction in P(2HB-co-LA) increased proportional to the concentration of sodium 2-hydroxybutyrate added to the culture medium. P(2HB-co-LA)] could also be produced from glucose as a sole carbon source without sodium 2-hydroxybutyrate into the culture medium. Recombinant E. coli XL1-Blue strain expressing the phaC1437, pct540, cimA3.7, and leuBCD genes together with the L. lactis Il1403 panE gene, successfully produced P(23.5mol%2HB-co-76.5mol%LA)] to the polymer content of 19.4 wt% when it cultured in a chemically defined medium containing 20 g/L of glucose. The metabolic engineering strategy reported here should be useful for the production of novel copolymer P(2HB-co-LA)].

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Volume21
Issue number1
DOIs
StatePublished - 1 Jan 2016

Bibliographical note

Funding Information:
This work was supported by the Technology Development Program to Solve Climate Changes (Systems Metabolic Engineering for Biorefineries) (NRF-2015M1A2A2035810) from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea and the Basic Science Research Program through the NRF funded by the Ministry of Education (NRF- 2013R1A1A2058379).

Publisher Copyright:
© 2016, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.

Keywords

  • 2-hydroxyacid
  • 2-hydroxybutyrate
  • PHA
  • lactate
  • poly(2-hydroxybutyrate-co-lactate)
  • recombinant E. coli

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