Abstract
Here, Corynebacterium glutamicum ATCC13032 expressing Baeyer–Villiger monooxygenase from Pseudomonas putida KT2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. Diverse parameters including cultivation and reaction temperatures, type of detergent, and pH were found to improve biotransformation efficiency. The optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant C. glutamicum was 15 °C, but the reaction temperature was optimal at 30 °C. Enhanced conversion efficiency was obtained by supplying 0.05 g/L of Tween 80 at pH 7.5. Under these optimal conditions, recombinant C. glutamicum produced 0.28 mM of 9-(nonanoyloxy) nonanoic acid with a 75.6% (mol/mol) conversion yield in 2 h. This is the first report on the biotransformation of 10-ketostearic acid to 9-(nonanoyloxy) nonanoic acid with a recombinant whole-cell C. glutamicum-based biocatalyst and the results demonstrate the feasibility of using C. glutamicum as a whole-cell biocatalyst.
Original language | English |
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Pages (from-to) | 1301-1311 |
Number of pages | 11 |
Journal | Journal of Industrial Microbiology and Biotechnology |
Volume | 44 |
Issue number | 9 |
DOIs | |
State | Published - 1 Sep 2017 |
Bibliographical note
Funding Information:This research was supported by the MOTIE/KEIT R&D Program (No. 10044604, Bioproduction of long chain dicarboxylic acids from fatty acids and lipids) and C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2015M3D3A1A01064929).
Publisher Copyright:
© 2017, Society for Industrial Microbiology and Biotechnology.
Keywords
- 10-Ketostearic acid
- 9-(Nonanoyloxy) nonanoic acid
- Biocatalyst
- Corynebacterium glutamicum