Bioprocess engineering to produce 10-hydroxystearic acid from oleic acid by recombinant Escherichia coli expressing the oleate hydratase gene of Stenotrophomonas maltophilia

Eun Yeong Jeon, Jung Hoo Lee, Kyung Mi Yang, Young Chul Joo, Deok Kun Oh, Jin Byung Park

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Microbial hydroxylation of long chain fatty acids has been extensively investigated. However, biotransformation productivity remains below ca. 1.0 g/g cell dry weight (CDW)/h under process conditions. In the present study, a highly efficient microbial hydroxylation process to convert oleic acid into 10-hydroxystearic acid was developed. A recombinant Escherichia coli expressing ohyA, the gene encoding oleate hydratase of Stenotrophomonas maltophilia, was used as the biocatalyst. Investigation of the ohyA expression and biotransformation conditions (e.g., inducer concentration, gene expression period before initiating biotransformation, mixing condition of reaction medium) enabled 10-hydroxystearic acid to accumulate to a final concentration of approximately 46 g/L in the culture medium. The specific product formation rate and product yield reached approximately 2.0 g/g CDW/h (i.e., 110 U/g CDW) and 91%, respectively. The specific product formation rate was more than 3-fold higher than those of a bioprocess using wild type Stenotrophomonas sp. cells. Additionally, the product of the whole-cell biotransformation was recovered at a yield of 70.9% and a purity of 99.7% via solvent fraction crystallization at low temperature. These results will contribute to developing a biological process for hydroxylation of oleic acid.

Original languageEnglish
Pages (from-to)941-947
Number of pages7
JournalProcess Biochemistry
Volume47
Issue number6
DOIs
StatePublished - Jun 2012

Keywords

  • 10-Hydroxystearic acid
  • Biotransformation
  • Escherichia coli
  • Oleate hydratase
  • Stenotrophomonas maltophilia

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