TY - JOUR
T1 - Biomimetic nanofibrous scaffolds
T2 - Preparation and characterization of chitin/silk fibroin blend nanofibers
AU - Park, Ko Eun
AU - Jung, Sung Youn
AU - Lee, Seung Jin
AU - Min, Byung Moo
AU - Park, Won Ho
N1 - Funding Information:
This work was supported by a grant from the Korean Ministry of Commerce, Industry, and Energy and by a grant from the Korean Science and Engineering Foundation through the Intellectual Biointerface Engineering Center at Seoul National University.
PY - 2006/5/30
Y1 - 2006/5/30
N2 - Electrospinning of chitin/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was investigated to fabricate a biomimetic nanostructured scaffolds for tissue engineering. The morphology of the electrospun chitin/SF blend nanofibers was investigated with a field emission scanning electron microscope (FE-SEM). The average diameters of chitin/SF blend fibers decreased from 920 to 340 nm, with the increase of chitin content in blend compositions. The miscibility of chitin/SF blend fibers was examined by solution viscosity measurement. The chitin and SF were immiscible in the as-spun nanofibrous structure. The dimensional stability of chitin/SF blend nanofibers, with or without water vapor after-treatment, was conducted by immersing in water. As-spun SF-rich blend nanofibrous matrices were lost their fibrous structure after the water immersion for 24 h, and then changed into membrane-like structure. On the contrary, nanofibrous structures of water vapor-treated SF-rich blends were almost maintained. To assay the cytocompatibility and cell behavior on the chitin/SF blend nanofibrous scaffolds, cell attachment and spreading of normal human epidermal keratinocyte and fibroblasts seeded on the scaffolds were studied. Our results indicate that chitin/SF blend nanofibrous matrix, particularly the one that contained 75% chitin and 25% SF, could be a potential candidate for tissue engineering scaffolds because it has both biomimetic three-dimensional structure and an excellent cell attachment and spreading for NHEK and NHEF.
AB - Electrospinning of chitin/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was investigated to fabricate a biomimetic nanostructured scaffolds for tissue engineering. The morphology of the electrospun chitin/SF blend nanofibers was investigated with a field emission scanning electron microscope (FE-SEM). The average diameters of chitin/SF blend fibers decreased from 920 to 340 nm, with the increase of chitin content in blend compositions. The miscibility of chitin/SF blend fibers was examined by solution viscosity measurement. The chitin and SF were immiscible in the as-spun nanofibrous structure. The dimensional stability of chitin/SF blend nanofibers, with or without water vapor after-treatment, was conducted by immersing in water. As-spun SF-rich blend nanofibrous matrices were lost their fibrous structure after the water immersion for 24 h, and then changed into membrane-like structure. On the contrary, nanofibrous structures of water vapor-treated SF-rich blends were almost maintained. To assay the cytocompatibility and cell behavior on the chitin/SF blend nanofibrous scaffolds, cell attachment and spreading of normal human epidermal keratinocyte and fibroblasts seeded on the scaffolds were studied. Our results indicate that chitin/SF blend nanofibrous matrix, particularly the one that contained 75% chitin and 25% SF, could be a potential candidate for tissue engineering scaffolds because it has both biomimetic three-dimensional structure and an excellent cell attachment and spreading for NHEK and NHEF.
KW - Blend nanofiber
KW - Chitin
KW - Electrospinning
KW - Fibroblast
KW - Matrix
KW - Silk fibroin
UR - http://www.scopus.com/inward/record.url?scp=33646673846&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2006.03.003
DO - 10.1016/j.ijbiomac.2006.03.003
M3 - Article
C2 - 16581120
AN - SCOPUS:33646673846
SN - 0141-8130
VL - 38
SP - 165
EP - 173
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
IS - 3-5
ER -